文献类型： 外文期刊

第一作者： Li, Shenghui

作者： Li, Shenghui;Wu, Chenglong;Li, Shenghui;Yang, Hongwei;Jin, Ya;Hao, Qiang;Zhou, Zhigang;Yang, Hongwei;Li, Shengkang;Liu, Shubin;Ding, Qianwen;Yao, Yuanyuan;Yang, Yalin;Ran, Chao;Zhang, Zhen;Yao, Yuanyuan;Zhou, Zhigang;Cheng, Kaimin;Hu, Jun;Liu, Hongliang;Zhou, Zhigang;Zhang, Zhen

作者机构： Huzhou Univ, Coll Life Sci, Zhejiang Prov Key Lab Aquat Bioresource Conservat, Nation Local Joint Engn Lab Aquat Anim Genet Breed, Huzhou 313000, Peoples R China;Chinese Acad Agr Sci, Feed Res Inst, China Norway Joint Lab Fish Gut Microbiota, Beijing 100081, Peoples R China;Shantou Univ, Inst Marine Sci, Shantou 515063, Peoples R China;Chinese Acad Agr Sci, Key Lab Feed Biotechnol, Minist Agr & Rural Affairs, Feed Res Inst, Beijing 100081, Peoples R China;Chinese Acad Agr Sci, Kunpeng Inst Modern Agr Foshan, Foshan 528225, Peoples R China;Guangdong Yuehai Feeds Grp Co Ltd, Zhanjiang 524017, Peoples R China;Chinese Acad Agr Sci, Inst Feed Res, China Norway Joint Lab Fish Gut Microbiota, Beijing 100081, Peoples R China;Chinese Acad Agr Sci, Inst Feed Res, Key Lab Feed Biotechnol, Minist Agr & Rural Affairs, Beijing 100081, Peoples R China

关键词： Gut microbiota; High fat diet; Homeostasis; Liver; Postbiotics

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 2022影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 142 卷

页码：

收录情况： SCI

摘要： Postbiotics have the ability to improve host metabolic disorders and immunity. In order to explore whether the postbiotics SWFC (cultured supernatant mixture of Cetobacterium somerae and Lactococcus lactis) repaired the adverse effects caused by feeding of high-fat diet (HFD), zebrafish were selected as the experimental animal and fed for 6 weeks, with dietary HFD as the control group, and HFD containing 0.3 g/kg and 0.4 g/kg SWFC as the treatment groups. The results indicated that addition of SWFC in the diet at a level of 0.3 and 0.4 g/kg didn't affect the growth performance of zebrafish (P > 0.05). Supplementation of dietary SWFC0.3 relieved lipid metabolism disorders through significant increasing in the expression of ppar alpha and cpt1, and decreasing the expression of cebp alpha, ppar gamma, acc1 and dgat-2 genes (P < 0.05). Moreover, the content of triacylglycerol was markedly lower in the liver of zebrafish grouped under SWFC0.3 (P < 0.05). Dietary SWFC0.3 also improved the antioxidant capacity via increasing the expression level of ho-1, sod and gstr genes, and significant inducing malondialdehyde content in the liver of zebrafish (P < 0.05). Besides, dietary SWFC0.3 also notably improved the expression level of lysozyme, c3a, defbl1 and defbl2 (P < 0.05). The expression level of pro-inflammatory factors (nf-kappa b, tnf-alpha, and il-1 beta) were significantly decreased and the expression level of anti-inflammatory fac-tor (il-10) was markedly increased in the postbiotics 0.3 g/kg group (P < 0.05). Feeding with SWFC0.3 sup-plemented diet for 6 weeks improved the homeostasis of gut microbiota and increased the survival rate of zebrafish after challenged with Aeromonus veronii Hm091 (P < 0.01). It was worth noting that the positive effect of dietary SWFC at a level of 0.3 g/kg was considerably better than that of 0.4 g/kg. This may imply that the effectiveness and use of postbiotics is limited by dosage.

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