A Nectin1 Mutant Mouse Model Is Resistant to Pseudorabies Virus Infection

文献类型： 外文期刊

第一作者： Yang, Xiaohui

作者： Yang, Xiaohui;Yu, Chuanzhao;Zhang, Qiuyan;Hong, Linjun;Gu, Ting;Zheng, Enqin;Xu, Zheng;Li, Zicong;Song, Changxu;Cai, Gengyuan;Wu, Zhenfang;Yang, Huaqiang;Yang, Xiaohui;Yu, Chuanzhao;Zhang, Qiuyan;Hong, Linjun;Gu, Ting;Zheng, Enqin;Xu, Zheng;Li, Zicong;Song, Changxu;Cai, Gengyuan;Wu, Zhenfang;Yang, Huaqiang

作者机构：

关键词： antiviral breeding; genetic modification; nectin1; pig; PRV; disease resistance

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 5 期

页码：

收录情况： SCI

摘要： The present study generated nectin1-mutant mice with single amino acid substitution and tested the anti-pseudorabies virus (PRV) ability of the mutant mice, with the aim to establish a model for PRV-resistant livestock. A phenylalanine to alanine transition at position 129 (F129A) of nectin1 was introduced into the mouse genome to generate nectin1 (F129A) mutant mice. The mutant mice were infected with a field-isolated highly virulent PRV strain by subcutaneous injection of virus. We found that the homozygous mutant mice had significantly alleviated disease manifestations and decreased death rate and viral loading in serum and tissue compared with heterozygous mutant and wild-type mice. In addition to disease resistance, the homozygous mutant mice showed a defect in eye development, indicating the side effect on animals by only one amino acid substitution in nectin1. Results demonstrate that gene modification in nectin1 is an effective approach to confer PRV resistance on animals, but the mutagenesis pattern requires further investigation to increase viral resistance without negative effect on animal development.

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