Genetic analysis and QTL mapping for silique density in rapeseed (Brassica napus L.)

文献类型： 外文期刊

第一作者： Ma, Xingrong

作者： Ma, Xingrong;Wang, Jinhua;Gu, Yongfen;Fang, Pengpeng;Nie, Wenjing;Luo, Ruirui;Liu, Jin;Qian, Wei;Mei, Jiaqin;Ma, Xingrong;Gu, Yongfen;Nie, Wenjing;Luo, Ruirui;Qian, Wei;Mei, Jiaqin;Wang, Jinhua;Fang, Pengpeng;Fang, Pengpeng;Fang, Pengpeng;Liu, Jin

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.4； 五年影响因子：5.7 ）

ISSN： 0040-5752

年卷期： 2023 年 136 卷 6 期

页码：

收录情况： SCI

摘要： Key messageGenetic models, QTLs and candidate gene for silique density on main inflorescence of rapeseed were identified.Silique density is one of the critical factors to determine seed yield and plant architecture in rapeseed (Brassica napus L.); however, the genetic control of this trait is largely unknown. In this study, the genetic model for silique density on main inflorescence (SDMI) of rapeseed was estimated according to the phenotypic data of P-1 (an inbreed line with high SDMI), P-2 (an inbreed line with low SDMI), F-1, F-2, BC1P1 and BC1P2 populations, revealing that SDMI is probably controlled by multi-minor genes with or without major gene. The QTLs for SDMI and its component characters including silique number on main inflorescence (SNMI) and main inflorescence length (MIL) were consequently mapped from a DH population derived from P-1 and P-2 by using a genetic linkage map constructed by restriction site-associated DNA sequencing (RAD seq) technology. A total of eight, 14 and three QTLs were identified for SDMI, SNMI and MIL under three environments, respectively, with an overlap among SDMI and SNMI in 55.7-75.4 cm on linkage group C06 which corresponding to 11.6-27.3 Mb on chromosome C06. Genomic resequencing was further conducted between a high- and a low-SDMI pool constructed from the DH population, and QTL-seq analysis identified a 0.15 Mb interval (25.98-26.13 Mb) from the C06-QTL region aforementioned. Transcriptome sequencing and qRT-PCR identified one possible candidate gene (BnARGOS) from the 0.15 Mb interval. This study will provide novel insights into the genetic basis of SD in rapeseed.

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