Expression pattern and tissue localization of the class B scavenger receptor BmSCRBQ4 in Bombyx mori

文献类型： 外文期刊

第一作者： Dong, Zhan-Peng

作者： Dong, Zhan-Peng;Huang, Ping;Liao, Peng-Fei;Liu, Min;Dai, Fang-Yin;Pan, Min-Hui;Lu, Cheng;Chai, Chun-Li;Dai, Fang-Yin;Pan, Min-Hui;Lu, Cheng;Wang, Wei;Lu, Cheng

作者机构：

关键词： BmSCRBQ4;Bombyx mori;Cameo2;class B scavenger receptor;gene expression;immunohistochemistry

期刊名称：INSECT SCIENCE （ 影响因子：3.262； 五年影响因子：3.206 ）

ISSN： 1672-9609

年卷期： 2015 年 22 卷 6 期

页码：

收录情况： SCI

摘要： Class B scavenger receptors (SR-Bs) are cell surface glycoproteins involved in various physiological processes in vivo, including the transport and metabolism of lipids, binding and phagocytosis of xenobiotics, and signaling. But little information is available about silkworm SR-Bs; it is necessary to study these SR-Bs for revealing their function. In this study, we cloned the full-length coding sequence of BmSCRBQ4, a SR-B gene from the silkworm Bombyx mori L. We found that the BmSCRBQ4 gene consists of nine exons and eight introns, with an open reading frame of 1371 bp encoding 456 amino acids. Gene expression studies determined that BmSCRBQ4 messenger RNA (mRNA) was expressed in unfertilized eggs, during embryonic development and throughout the majority of the larval period. Expression of mRNA was detected in the mid gut, middle silk gland, posterior silk gland, head, integumentum, fat body, testes and the ovaries of the larval B. mori Dazao strain, as well as in the silkworm cell lines BmN and BmE. Protein expression studies found BmSCRBQ4 protein was expressed only in the testes, fat body and middle silk gland of larvae, as well as in the silkworm cell lines BmN and BmE. The BmSCRBQ4 protein showed variability in banding patterns in different tissues and cells when analyzed by Western blotting. Immunohistochemical staining showed that the BmSCRBQ4 protein localizes to the constitutive membranes or cellular membranes of these tissues. These results indicated that BmSCRBQ4 gene may play some physiologically relevant roles at the cell surface in each tissue.

分类号：

  • 相关文献

[1]Cloning, Expression Feature, and Tissue Location of BmSCRBQ1, a Class B Scavenger Receptor in Bombyx mori (L.). Dong, Z. P.,Chai, C. L.,Dai, F. Y.,Pan, M. H.,Lu, C.,Dong, Z. P.,Chai, C. L.,Dai, F. Y.,Pan, M. H.,Lu, C.,Wang, W.,Lu, C.. 2013

[2]Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Liu, JM,Cai, XZ,Lin, JJ,Fu, ZQ,Yang, GZ,Shi, FH,Cai, YM,Shen, W,Taylor, MG,Wu, XF. 2004

[3]Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm, Bombyx mori. Zhao, Qiaoling.

[4]SAGE tag based cDNA microarray analysis during larval to pupal development and isolation of novel cDNAs in Bombyx mori. Zhang, Yong,Huang, Jianhua,Jia, Shihai,Liu, Wenbin,Li, Muwang,Wang, Sibao,Miao, Xuexia,Xiao, Huasheng,Huang, Yongping. 2007

[5]SSR marker-based mapping and linkage analysis of Bombyx mori thermotolerance gene. Zhao, Yuan,Wu, Yang-Chun,Zhu, Ya-Hong,Zhao, Yuan,Zhang, Jiang.

[6]Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain. Wang, Xi,Hamid, Mohammod Abdul,Zhao, Xingbo,Wang, Xi,Li, Hongxia. 2012

[7]Immunolocalization of estrogen receptor alpha in Neomysis japonica oocytes and follicle cells during ovarian development. Yang, Xiaozhen,Zhao, Liulan,Zhao, Zhanzhong,Hu, Bing,Wang, Chun,Yang, Zhigang,Cheng, Yongxu,Zhao, Zhanzhong. 2012

[8]Detection methods of Cyprinid herpesvirus 2 infection in silver crucian carp (Carassius auratus gibelio) via a pORF72 monoclonal antibody. Kong, S. Y.,Jiang, Y. S.,Lu, J. F.,Xu, D.,Lu, L. Q.,Wang, Q..

[9]Different toxicity of the novel Bacillus thuringiensis (Bacillales: Bacillaceae) strain LLP29 against Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae). Zhang, Lingling,Tang, Baozhen,Huang, Zhipeng,Liu, Zhaoxia,Huang, Tianpei,Guan, Xiong,Huang, Enjiong,Gelbic, Ivan,Xu, Lei.

[10]Expression pattern analysis of odorant-binding proteins in the pea aphid Acyrthosiphon pisum. Riviello, Lea,Falabella, Patrizia,Bruno, Daniele,Grimaldi, Annalisa,Congiu, Terenzio,Sun, Yu Feng. 2015

[11]Expression and Prognostic Values of Id-1 and Id-3 in Gastric Adenocarcinoma. Yang, Hai-Yan,Liu, Lian-Xin,Jiang, Hong-Chi,Liu, Hao-Ling,Zhu, Hong,Liu, Gai-Yun,Meng, Qing-Wen,Qu, Lian-Dong. 2011

[12]Wnt5a as a Predictor in Poor Clinical Outcome of Patients and a Mediator in Chemoresistance of Ovarian Cancer. Peng, Chunjie,Zhang, Xiaolei,Yu, Hongli,Zheng, Jianhua,Wu, Donglai. 2011

[13]Characterization and application of monoclonal antibodies against Mycoplasma hyorhinis pyruvate dehydrogenase E1 complex subunit alpha. Chen, Dongjie,Wei, Yanwu,Huang, Liping,Wang, Yiping,Du, Wenjuan,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[14]Molecular characterization and tissue localization of sensory neuron membrane protein from Chinese honey bee, Apis cerana cerana (Hymenoptera: Apidae). Hu, Ying-Ying,Xu, Shu-Fa,Wubie, Abebe Jenberie,Li, Wei,Huang, Jia-Xing,Guo, Zhan-Bao,Zhou, Ting,Wubie, Abebe Jenberie. 2013

[15]Expression and prognostic value of ING3 in human primary hepatocellular carcinoma. Yang, Hai-Yan,Tian, Lan-Tian,Song, Rui-Peng,Song, Xuan,Yin, Da-Long,Liang, Ying-Jian,Jiang, Hong-Chi,Liu, Lian-Xin,Liu, Hao-Ling,Qu, Lian-Dong,Liu, Jia-Ren.

[16]Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system. Gregson, Norman A.,Zhang, Gaiping,Pritchard, Jane,Wang, Aiping,Sanvito, Lara,Hayday, Adrian C.,Hughes, Richard A. C.. 2007

[17]Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis. Tan, Deguan,Sun, Xuepiao,Zhang, Jiaming. 2011

[18]Characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker Larimichthys crocea. Zhang, Dong Ling,Lv, Chang Huan,Wang, Zhi Yong,Yu, Da Hui.

[19]Susceptibility of farmed juvenile giant grouper Epinephelus lanceolatus to a newly isolated grouper iridovirus (genus Ranavirus). Peng, Chao,Ma, Hongling,Su, Youlu,Wen, Weigeng,Feng, Juan,Guo, Zhixun,Qiu, Lihua,Peng, Chao.

[20]Increased expression of CSP and CYP genes in adult silkworm females exposed to avermectins. Xuan, Ning,Guo, Xia,Xie, Hong-Yan,Liu, Guo-Xia,Picimbon, Jean-Francois,Lou, Qi-Nian,Lu, Xing-Bo. 2015

作者其他论文 更多>>

微信小程序