Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA)
文献类型: 外文期刊
第一作者: Li, Y
作者: Li, Y;Xi, YW;Zhang, ZK;Huang, XQ
作者机构:
关键词: chalcone synthase A;co-suppression;Petunia hybrida;in situ hybridization
期刊名称:SCIENCE IN CHINA SERIES C-LIFE SCIENCES ( 影响因子:1.61; 五年影响因子:1.148 )
ISSN: 1006-9305
年卷期: 2001 年 44 卷 6 期
页码:
收录情况: SCI
摘要: Chalcone synthase A is a key enzyme in the anthocyanin biosynthesis pathway. Expression of chsA gene in transgenic Petunia hybrida resulted in flower color alterations and co-suppression of transgenes and endogenous genes. We fused the a-glucuronidase (uidA) gene to the C-terminal of chsA gene, and transferred the fusion gene into Petunia hybrida via Agrobacterium tumefaciens. GUS histochemical staining analysis showed that co-suppression occurred specifically during the development of flowers and co-suppression required the mutual interaction of endogenous genes and transgenes. RNA in situ hybridization analysis suggested that co-suppression occurred in the entire plant, and RNA degradation occurred in the cytoplasm.
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