Construction of a multicontrol sterility system for a maize male-sterile line and hybrid seed production based on the ZmMs7 gene encoding a PHD-finger transcription factor

文献类型： 外文期刊

第一作者： Zhang, Danfeng

作者： Zhang, Danfeng;Liu, Shuangshuang;Rao, Liqun;Wan, Xiangyuan;Zhang, Danfeng;Wu, Suowei;An, Xueli;Xie, Ke;Dong, Zhenying;Zhu, Taotao;Wan, Xiangyuan;Xu, Liwen;Zhao, Jiuran;Zhang, Danfeng;Zhou, Yan;Fang, Wen;Liu, Shensi;Liu, Shuangshuang;Li, Jinping

作者机构：

关键词： ZmMs7;genetic male sterility;hybrid seed production;transgenic maintainer line;nontransgenic progeny;maize

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：9.803； 五年影响因子：9.555 ）

ISSN： 1467-7644

年卷期： 2018 年 16 卷 2 期

页码：

收录情况： SCI

摘要： Although hundreds of genetic male sterility (GMS) mutants have been identified in maize, few are commercially used due to a lack of effective methods to produce large quantities of pure male-sterile seeds. Here, we develop a multicontrol sterility (MCS) system based on the maize male sterility 7 (ms7) mutant and its wild-type Zea mays Male sterility 7 (ZmMs7) gene via a transgenic strategy, leading to the utilization of GMS in hybrid seed production. ZmMs7 is isolated by a map-based cloning approach and encodes a PHD-finger transcription factor orthologous to rice PTC1 and Arabidopsis MS1. The MCS transgenic maintainer lines are developed based on the ms7-6007 mutant transformed with MCS constructs containing the (i)ZmMs7 gene to restore fertility, (ii) -amylase gene ZmAA and/or (iii) DNA adenine methylase gene Dam to devitalize transgenic pollen, (iv) red fluorescence protein gene DsRed2 or mCherry to mark transgenic seeds and (v) herbicide-resistant gene Bar for transgenic seed selection. Self-pollination of the MCS transgenic maintainer line produces transgenic red fluorescent seeds and nontransgenic normal colour seeds at a 1:1 ratio. Among them, all the fluorescent seeds are male fertile, but the seeds with a normal colour are male sterile. Cross-pollination of the transgenic plants to male-sterile plants propagates male-sterile seeds with high purity. Moreover, the transgene transmission rate through pollen of transgenic plants harbouring two pollen-disrupted genes is lower than that containing one pollen-disrupted gene. The MCS system has great potential to enhance the efficiency of maize male-sterile line propagation and commercial hybrid seed production.

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