Molecular cloning, expression, and functional features of IGF1 splice variants in sheep

文献类型： 外文期刊

第一作者： Song, Xu-Ting

作者： Song, Xu-Ting;Zhang, Jia-Nan;Zhao, Duo-Wei;Zhai, Yu-Fei;Lu, Qi;Yang, Xiu-Qin;Yao, Yu-Chang;Qi, Mei-Yu;Lu, Ming-Hai;Deng, Shou-Long;Han, Hong-Bing

作者机构：

关键词： sheep; IGF1; splice variants; tissue expression; proliferation; apoptosis

期刊名称：ENDOCRINE CONNECTIONS （ 影响因子：3.335； 五年影响因子：3.489 ）

ISSN：

年卷期： 2021 年 10 卷 9 期

页码：

收录情况： SCI

摘要： Insulin-like growth factor 1 (IGF1), also known as somatomedin C, is essential for the regulation of animal growth and development. In many species, the IGF1 gene can be alternatively spliced into multiple transcripts, encoding different pre-pro-IGF1 proteins. However, the exact alternative splicing patterns of IGF1 and the sequence information of different splice variants in sheep are still unclear. In this study, four splice variants (class 1-Ea, class 1-Eb, class 2-Ea, and class 2-Eb) were obtained, but no IGF1 Ec, similar to that found in other species, was discovered. Bioinformatics analysis showed that the four splice variants shared the same mature peptide (70 amino acids) and possessed distinct signal peptides and E peptides. Tissue expression analysis indicated that the four splice variants were broadly expressed in all tested tissues and were most abundantly expressed in the liver. In most tissues and stages, the expression of class 1-Ea was highest, and the expression of other splice variants was low. Overall, levels of the four IGF1 splice variants at the fetal and lamb stages were higher than those at the adult stage. Overexpression of the four splice variants significantly increased fibroblast proliferation and inhibited apoptosis (P < 0.05). In contrast, silencing IGF1 Ea or IGF1 Eb with siRNA significantly inhibited proliferation and promoted apoptosis (P < 0.05). Among the four splice variants, class 1-Ea had a more evident effect on cell proliferation and apoptosis. In summary, the four ovine IGF1 splice variants have different structures and expression patterns and might have different biological functions.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序