Transcriptome analysis of rosette and folding leaves in Chinese high-throughput RNA sequencing

文献类型： 外文期刊

第一作者： Wang, Fengde

作者： Wang, Fengde;Li, Libin;Li, Huayin;Liu, Lifeng;Zhang, Yihui;Gao, Jianwei;Wang, Xiaowu

作者机构：

关键词： Chinese cabbage; Rosette leaves; Folding leaves; Transcriptome; High-throughput RNA sequencing

期刊名称：GENOMICS （ 影响因子：5.736； 五年影响因子：4.939 ）

ISSN： 0888-7543

年卷期： 2012 年 99 卷 5 期

页码：

收录情况： SCI

摘要： In this study, we report the first use of RNA-sequencing to gain insight into the wide range of transcriptional events that are associated with leafy head development in Chinese cabbage. We generated 53.5 million sequence reads (90 bp in length) from the rosette and heading leaves. The sequence reads were aligned to the recently sequenced Chiifu genome and were analyzed to measure the gene expression levels, to detect alternative splicing events and novel transcripts, to determine the expression of single nucleotide polymorphism.;, and to refine the annotated gene structures. The analysis of the global gene expression pattern suggests two important concepts, which govern leafy head formation. Firstly, some stimuli, such as carbohydrate levels, light intensity and endogenous hormones might play a critical role in regulating the leafy head formation. Secondly, the regulation of transcription factors, protein kinases and calcium may also be involved in this developmental process. (C) 2012 Published by Elsevier Inc.

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