Identification and sequence analysis of aroA gene of avibacterium paragallinarum
文献类型: 外文期刊
第一作者: Lv, Xue-Ze
作者: Lv, Xue-Ze;Wang, Hong-Jun;Chen, Xiao-Ling;Gong, Yu-Mei;Liang, Yu-Rong;He, Yun-Xia;Zhang, Pei-Jun
作者机构:
关键词: Apg;aroA;gene identification;protein structure;molecule evolution
期刊名称:INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE ( 影响因子:0.166; 五年影响因子:0.621 )
ISSN: 1940-5901
年卷期: 2016 年 9 卷 2 期
页码:
收录情况: SCI
摘要: Objectives: A convenient and efficient method for the clone of unknown full-length gene was established and the structure and molecule evolution of full-length aroA gene of Avibacterium paragallinarum were evaluated. Methods: This experiment took an international standard strain 145 (C-3) of Apg as template, designed degenerate primers according to aroA gene with CODEHOP software. The improved chromosome walking was used to amplify the whole gene sequence of aroA. Some structure researches on the nucleicacid and coding protein sequence have been done. Besides, we also did some research on molecular evolution analysis with other serotypes and related bacteria by sequence alignment. Results: Complete aroA gene of Avibacterium paragallinarum was obtained, which is 1 293 bp. The length of the gene encoded product is 430aa, which have three transmembrane domains and five potential epitopes. The homology of amino acid sequences is 91.4%-100% between different serotypes experiment strains, and the nucleic acid homology is over 74.2% between bacteria of the same family. Conclusions: This trial has combined the degenerate PCR and improved chromosome walking for the research of avibacterium coli aroA genes complete sequence for the first time, and obtained the expected results. It laid the foundation for the study of biological activity of its encoded protein and building avibacterium bacilli aroA deletion attenuated strains.
分类号:
- 相关文献
作者其他论文 更多>>
-
Penaeus chinensis nearing a critical threshold of susceptibility to Decapod iridescent virus 1 (DIV1)
作者:Guo, Xiao-Meng;Wang, Guo-Hao;Xing, Jing-Yi;Qiu, Liang;Huang, Jie;Qiu, Liang;Huang, Jie;Yao, Jia-Yun;Guo, Xiao-Meng;Wang, Guo-Hao;Huang, Jie;Chen, Xiao-Ling;Xing, Jing-Yi
关键词:Decapod iridescent virus 1; Susceptible species; Penaeus chinensis; Non-invasive challenge mimicking nature; transmission pathway
-
A unique three-enzyme cascade mediates efficient regioselective and stereospecific epoxytetrahydrofuran ring formation in deoxyverrucosidin biosynthesis
作者:Wei, Hui-Ling;Chen, Xiao-Ling;Dai, Yu;Li, Shu-Ming;Yang, Li
关键词:
-
Intelligent Bamboo Part Sorting System Design via Machine Vision
作者:Liu, Tian-Hu;Wu, Zi-Di;Nie, Xiang-Ning;Li, Gui-Qi;Wang, Hong-Jun;Zhang, Di;Liu, Wei;Wu, Jin-Meng;Chen, Qin-Ling
关键词:
-
Herbicidal activities of compounds isolated from the medicinal plant Piper sarmentosum
作者:Feng, Gang;Chen, Min;Ye, Huo-Chun;Li, Hong;Chen, Li-Liang;Chen, Xiao-Ling;Yan, Chao;Zhang, Jing;Feng, Gang;Ye, Huo-Chun;Chen, Li-Liang;Chen, Xiao-Ling;Yan, Chao;Zhang, Jing;Chen, Min;Zhang, Zheng-Ke;Li, Hong
关键词:Piper sarmentosum; Sarmentosine; Sarmentine; Herbicidal activity
-
Oxidative damage and antioxidative indicators in 48 h germinated rice embryos during the vitrification-cryopreservation procedure
作者:Huang, Bin;Zhou, Yuan-Chang;Huang, Bin;Zhang, Jin-Mei;Chen, Xiao-Ling;Xin, Xia;Yin, Guang-Kun;He, Juan-Juan;Lu, Xin-Xiong;Huang, Bin;Zhou, Yuan-Chang
关键词:Embryo; Rice; Apical meristem; Reactive oxygen species; Stress; Vitrification
-
Cryopreservation of Citrus anthers in the National Crop Genebank of China
作者:Zhang, Jin-Mei;Lu, Xin-Xiong;Xin, Xia;Yin, Guang-Kun;He, Juan-Juan;Huang, Bin;Chen, Xiao-Ling;Huang, Bin;Jiang, Dong
关键词:Citrus;Air-desiccation;Cryopreservation;In vitro germination;Pollen
-
CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD
作者:Zhang, Jin-mei;Lu, Xin-xiong;Xin, Xia;Yin, Guang-kun;He, Juan-juan;Chen, Xiao-ling;Huang, Bin;Volk, Gayle M.
关键词:Atractylodes macrocephala Koidz.;droplet-vitrification;regrowth;shoot tips;survival;thermal analysis