CRYOPRESERVATION OF IN VITRO-GROWN SHOOT TIPS OF CHINESE MEDICINAL PLANT Atractylodes macrocephala KOIDZ. USING A DROPLET-VITRIFICATION METHOD
文献类型: 外文期刊
第一作者: Zhang, Jin-mei
作者: Zhang, Jin-mei;Lu, Xin-xiong;Xin, Xia;Yin, Guang-kun;He, Juan-juan;Chen, Xiao-ling;Huang, Bin;Volk, Gayle M.
作者机构:
关键词: Atractylodes macrocephala Koidz.;droplet-vitrification;regrowth;shoot tips;survival;thermal analysis
期刊名称:CRYOLETTERS ( 影响因子:1.066; 五年影响因子:1.0 )
ISSN: 0143-2044
年卷期: 2015 年 36 卷 3 期
页码:
收录情况: SCI
摘要: BACKGROUND: Atractylodes macrocephala Koidz. is an important medicinal species from China that has been used for thousands of years for its special pharmacological antioxidant, hepatoprotective, anti-inflammatory, anti-allergic, antithrombotic, antiviral, and anticarcinogenic activities. OBJECTIVE: The aim of this research was to develop an efficient droplet-vitrification protocol for A. macrocephala shoot tips which could be used as a strategy for long-term conservation within gene banks. MATERIALS AND METHODS: The duration of preculture, loading, and PVS2 steps, as well as the recovery medium formulation, were optimized to achieve high levels of survival and regrowth for A. macrocephala shoot tips after liquid nitrogen exposure. RESULTS: Survival and regrowth levels after cryopreservation in the cultivar 'Baizhu' were as high as 76% and 62%, respectively. Thermal analysis using differential scanning calorimetry suggested that the PVS2 treatment plays a critical role for successful cryopreservation. CONCLUSION: The droplet-vitrification method established in this study could be used to cryopreserve A. macrocephala.
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