Development of SCAR Markers to Determine the Mating Types of Lepista nuda Protoplast Monokaryons

文献类型： 外文期刊

第一作者： Li, Dengjin

作者： Li, Dengjin;Liu, Yu;Wang, Peng;Ma, Yuanwei;Wang, Shouxian;Zhao, Shuang;Xu, Feng;Li, Dengjin

作者机构：

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN： 0343-8651

年卷期： 2014 年 68 卷 4 期

页码：

收录情况： SCI

摘要： Lepista nuda (Bull. ex Fr.) Cooke belongs to Tricholomataceae and is an edible fungus with both economic and medical value. Mycelia were isolated from the fruiting bodies of L. nuda and were used to prepare the protoplast monokaryons. One hundred and fifteen monokaryons were obtained and their mating types were determined using somatic incompatibility tests. Protoplast monokaryons segregated into either the A(1)B(1) or the A(2)B(2) mating types. Inter-simple sequence repeats and sequence-related amplified polymorphism fingerprinting were used to analyse the mating types of these protoplast monokaryons and 16 sequence-characterised amplified region primers were developed to efficiently differentiate between the monokaryon mating types. Multiplex PCR analyses were also established. The data presented here outline a method for the precise and rapid identification of protoplast monokaryon mating types, which has the promise to shorten the period required for conventional crossbreeding.

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