Chloroplast Simple Sequence Repeat (cp-SSR) Analysis for Determining the Origin of J-hopeiensis

文献类型： 外文期刊

第一作者： Qi, J. X.

作者： Qi, J. X.;Wu, C. L.;Dong, N. G.;Chen, Y. H.;Hao, Y. B.

作者机构：

关键词： J. hopeiensis;J. regia;J. mandshurica;origin;cp-SSR

期刊名称：VII INTERNATIONAL WALNUT SYMPOSIUM

ISSN： 0567-7572

年卷期： 2014 年 1050 卷

页码：

收录情况： SCI

摘要： To define the source of Ma walnut (J. hopeiensis), 10 chloroplast simple sequence repeat (cp-SSR) primers were used to amplify SSR fragments from 7 samples of J. regia, 8 samples of J. mandshurica, 34 samples of J. hopeiensis, and 1 hybid of J. mandshurica female xJ. regia male. The results showed that four cpSSR loci were polymorphic. According to alleles of all samples generated by the four polymorphic cpSSR loci, the genotypes of the hybrid of J. mandshurica female xJ. regia male and 24 samples of J. hopeiensis were the same to that of J. mandshurica Only 1 sample of J. hopeiensis named 'Yanqingmahetao-2' had the same genotype as J. regia. The genotypes of 9 samples of J. hopeiensis from Shaanxi were the same as that of J. mandshurica in 3 polymorphic cp-SSR loci, while they were the same as that of J. regia in 1 polymorphic cp-SSR locus. These results indicated that J. hopeiensis originated mainly from the natural hybrid of J. mandshurica female xJ. regia male. while 97.1% of J. hopeiensis came from the hybrid of J. mandshurica female xJ. regia male, while 3% of J. hopeiensis came from J. regia female xJ. mandshurica male in the wild population of J. hopeiensis. Furthermore, genetic difference in cp-SSR existed between J. hopeiensis from Shaanxi and those from Beijing and Hebei.

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