Forming coumarin C-glycosides via biocatalysis: Characterization of a C-glycosyltransferase from Angelica decursiva

文献类型： 外文期刊

第一作者： Wang, Zhiming

作者： Wang, Zhiming;Jiang, Xuefei;Wang, Zhiming;Jiang, Xuefei;He, Yuedong;Liao, Lijing;Xiao, Yibei;Zhang, Yuhao;Zhao, Yucheng;Qiao, Fei

作者机构：

关键词： Angelica decursiva; C-glycosyltransferase; Coumarin; Catalytic mechanism; Molecular docking

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.322； 五年影响因子：3.498 ）

ISSN： 0006-291X

年卷期： 2022 年 614 卷

页码：

收录情况： SCI

摘要： A glycosyl transferase, isolated from Angelica decursiva a medical herb rich in coumarin, shows C-glycosyl transferase activity by in vitro activity assay using 5,7-dihydroxycoumarin as substrate, producing a C-glycosylated product at position C ' 8 along with the main product at C ' 6 position. Catalytic promiscuity assay shows that AdCGT also displays O- or C-glycosylation activity to other coumarins and flavonoids. When phloretin and 2,4,6-trihydroxyacetophenone were fed as substrates, AdCGT catalyzed the formation of di-C-glycosides. Therefore, AdCGT is a multifunctional glycosyltransferase with a broad substrate acceptability. This work highlights the potential of AdCGT as a catalyst for glycosylation of coumarin and reveals a new regio-selective C-glycosyltransferase, providing a basis for exploring the mechanism of coumarin glycosylation. (c) 2022 Elsevier Inc. All rights reserved.

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