Expression Analysis of Anthocyanin Biosynthetic Genes in Different Colored Sweet Cherries (Prunus avium L.) During Fruit Development

文献类型： 外文期刊

第一作者： Liu, Yun

作者： Liu, Yun;Shen, Xinjie;Zhao, Kai;Guo, Xinwei;Li, Tianhong;Ben, Yue;Zhang, Xiaoming

作者机构：

关键词： Sweet cherries;Anthocyanin synthetic genes;qRT-PCR;Western blotting;Ultra-performance liquid chromatography

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN： 0721-7595

年卷期： 2013 年 32 卷 4 期

页码：

收录情况： SCI

摘要： To identify the key enzymes involved in anthocyanin synthesis in sweet cherry (Prunus avium L.), the differences in anthocyanin biosynthetic gene expressions were investigated in the samples (mix of peel and flesh) of a red-colored cultivar ('Hongdeng') and a bicolored cultivar ('Caihong') during fruit development. The expression of six anthocyanin synthetic genes in cherry (PacCHS, PacCHI, PacF3H, PacDFR, PacANS, and PacUFGT) was analyzed by quantitative real-time PCR and Western blot analysis. Meanwhile, the changes in anthocyanin contents were measured by ultra-performance liquid chromatography. The expression of anthocyanin synthetic genes and the anthocyanin contents were much higher in 'Hongdeng' than in 'Caihong' fruits. Gene transcription and translation and anthocyanin accumulation all started approximately at the end of the pit-hardening period and reached a maximum at maturation. All six genes were significantly correlated with anthocyanin accumulation in 'Hongdeng' and PacCHS had the highest direct effect. However, only PacUFGT was significantly correlated with anthocyanin accumulation in 'Caihong'. Anthocyanin biosynthesis in sweet cherry seems to be regulated mostly at the transcript levels. CHS appears to be the key enzyme involved in anthocyanin synthesis in 'Hongdeng', while UFGT is involved in anthocyanin synthesis in 'Caihong' fruits.

分类号：

  • 相关文献

[1]UPLC Quantitative Analysis of Multi-Components by Single Marker and Quality Evaluation of Polygala tenuifolia Wild. Extracts. Xu, Rui,Mao, Fuying,Zhao, Yunsheng,Wang, Wenping,Fan, Lingling,Gao, Xiaojuan,Zhao, Jianjun,Mao, Fuying,Zhao, Yunsheng,Wang, Wenping,Gao, Xiaojuan,Zhao, Jianjun,Zhao, Yunsheng,Tian, Hongling. 2017

[2]Influence of pH, concentration and light on stability of allicin in garlic (Allium sativum L.) aqueous extract as measured by UPLC. Wang, Haiping,Li, Xixiang,Liu, Xinyan,Shen, Di,Qiu, Yang,Zhang, Xiaohui,Song, Jiangping.

[3]Determination of neonicotinoid insecticides residues in eels using subcritical water extraction and ultra-performance liquid chromatography-tandem mass spectrometry. Xiao, Zhiming,Yang, Yunxia,Li, Yang,Fan, Xia,Ding, Shuangying.

[4]Analysis of free amino acids in Amur sturgeon by ultra-performance liquid chromatography using pre-column derivatization with 6-aminoquinolyl-carbamyl. Sun, Yanchun,Xu, Xianzhu,Sun, Yanchun,Mou, Zhenbo,Wu, Song,Wang, Jing,Tan, Zhijun.

[5]Simultaneous determination of pyrethrins residues in teas by ultra-performance liquid chromatography/tandem mass spectrometry. Liu, Xingang,Dong, Fengshou,Xu, Jun,Zhang, Changpeng,Li, Yuanbo,Zheng, Yongquan,Song, Wencheng.

[6]Ultra-performance LC Separation and Quadrupole Time-of-flight MS Identification of Major Alkaloids in Plumula Nelumbinis. Lin, Zongtao,Yang, Ruinan,Li, Wei,Guan, Zheng,Chen, Ailiang. 2014

[7]Genetic effect of an A/G polymorphism in the HSP70 gene on thermotolerance in chicken. Chen, Z. Y.,Zhang, W. W.,Gan, J. K.,Kong, L. N.,Zhang, X. Q.,Zhang, D. X.,Luo, Q. B.,Chen, Z. Y.,Zhang, W. W.,Gan, J. K.,Kong, L. N.,Zhang, X. Q.,Zhang, D. X.,Luo, Q. B.. 2016

[8]Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds. Lu Yun-feng,Zhang Bo,Li Qing-gang,Wang Zhi-xiu,Zhang Hao,Wu Ke-liang,Lu Yun-feng,Chen Ji-bao,Li Qing-gang. 2017

[9]Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein. Qi, Ting,Cui, Shang-jin.

[10]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[11]Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway. Gao, Hai-na,Hu, Han,Zheng, Nan,Wang, Jia-qi,Gao, Hai-na,Gao, Hai-na,Hu, Han,Zheng, Nan,Wang, Jia-qi,Gao, Hai-na,Hu, Han,Zheng, Nan,Wang, Jia-qi. 2015

[12]Changes of flavonol synthase and flavonol contents during grape berry development. Fang, Fang,Huang, Wei-Dong,Tang, Ke. 2013

[13]Expression and Prognostic Values of Id-1 and Id-3 in Gastric Adenocarcinoma. Yang, Hai-Yan,Liu, Lian-Xin,Jiang, Hong-Chi,Liu, Hao-Ling,Zhu, Hong,Liu, Gai-Yun,Meng, Qing-Wen,Qu, Lian-Dong. 2011

[14]A recently identified ovine Babesia in China: Serology and sero-epidemiology. Guan, Guiquan,Ma, Miling,Liu, Aihong,Ren, Qiaoyun,Wang, Jinming,Yang, Jifei,Li, Anyan,Liu, Zhijie,Du, Pengfei,Li, Youquan,Liu, Qing,Zhu, Hai,Yin, Hong,Luo, Jianxun.

[15]Valid application of western blotting. Hu, Xiuli,Chen, Yanhui,Wu, Liuji,Hu, Xiuli,Chen, Yanhui,Wu, Liuji,Chen, Yanhui,Tang, Haitao,Han, Zanping.

[16]Grass carp reovirus-GD108 fiber protein is involved in cell attachment. Tian, Yuanyuan,Jiao, Zhenzhen,Dong, Junjian,Sun, Chengfei,Jiang, Xiaoyan,Ye, Xing,Tian, Yuanyuan,Jiao, Zhenzhen.

[17]GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L). Fengli Zhao,JianhuiMa,Libei Li,Shuli Fan,Yaning Guo,Meizhen Song,Hengling Wei,Chaoyou Pang,Shuxun Yu. 2016

[18]SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40 degrees C water. Guan, Changfei,Chen, Li,Chen, Wenxing,Mo, Rongli,Zhang, Qinglin,Liu, Jihong,Luo, Zhengrong,Du, Xiaoyun. 2015

[19]A Novel AP2/ERF Transcription Factor CR1 Regulates the Accumulation of Vindoline and Serpentine in Catharanthus roseus. Gao, Fangyuan,Ren, Juansheng,Lu, Xianjun,Ren, Guangjun,Wang, Rui. 2017

[20]Molecular characterization and expression patterns of PsSVP genes reveal distinct roles in flower bud abortion and flowering in tree peony (Paeonia suffruticosa). Wang, Shunli,Xue, Jingqi,Zhu, Fuyong,Ren, Xiuxia,Zhang, Xiuxin,Wang, Shunli,Xue, Jingqi,Zhu, Fuyong,Ren, Xiuxia,Zhang, Xiuxin,Ahmadi, Noorollah,Holloway, Patricia. 2014

作者其他论文 更多>>

微信小程序