Study on Absorption, Distribution, Metabolism, and Excretion Properties of Novel Insecticidal GABA Receptor Antagonist, Pyraquinil, in Diamondback Moth Combining MALDI Mass Spectrometry Imaging and High-Resolution Mass Spectrometry

文献类型： 外文期刊

第一作者： Wan, Kai

作者： Wan, Kai;Xu, Hanhong;Wan, Kai;Xu, Hanhong;Wan, Kai;Jiang, Xunyuan;Tang, Xuemei;Xiao, Lu;Chen, Yan;Huang, Congling;Zhu, Fuwei;Wang, Fuhua;Wan, Kai;Jiang, Xunyuan;Tang, Xuemei;Xiao, Lu;Chen, Yan;Huang, Congling;Zhu, Fuwei;Wang, Fuhua

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关键词： pyraquinil; ADME; mass spectrometry imaging; Plutella xylostella

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.895； 五年影响因子：6.048 ）

ISSN： 0021-8561

年卷期： 2022 年 70 卷 20 期

页码：

收录情况： SCI

摘要： A thorough understanding of absorption, distribution, metabolism, and excretion (ADME) of insecticide candidates is essential in insecticide development and structural optimization. Here, ADME of pyraquinil, a novel insecticidal GABA receptorantagonist, inPlutella xylostellalarvae during the accumulation phase and depuration phase was investigated separately using acombination of UHPLC-Q-Orbitrap, HPLC-MS/MS, and MALDI-MSI. Five new metabolites of pyraquinil were identified, and ametabolic pathway was proposed. The oxidative metabolite (pyraquinil-sulfone) was identified as the main metabolite and confirmedby its standard. Quantitative results showed that pyraquinil was taken up by the larvae rapidly and then undergone a cytochromeP450s-mediated oxidative transformation into pyraquinil-sulfone.Both fecal excretion and oxidative metabolism were demonstratedto be predominant ways to eliminate pyraquinil inP. xylostellalarvae during accumulation, while oxidative metabolism followed byfecal excretion was probably the major pathway during depuration. MALDI-MSI revealed that pyraquinil was homogeneouslydistributed in the larvae, while pyraquinil-sulfone presented a continuous enrichment in the midgut during accumulation.Conversely, pyraquinil-sulfone located in hemolymph can be preferentially eliminated during depuration, suggesting its tissuetropism. It improves the understanding of the fate of pyraquinil inP. xylostellaand provides useful information for insecticidalmechanism elucidation and structural optimization of pyraquinil.

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