Effects of gibberellin mutations on in vitro shoot bud regeneration of Arabidopsis

文献类型： 外文期刊

第一作者： Zhang, Xiuhai

作者： Zhang, Xiuhai;Wu, Zhongyi;Huang, Conglin

作者机构：

关键词： Shoot bud regeneration;Arabidopsis thaliana;gibberellin;DELLA protein mutant

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2008 年 7 卷 22 期

页码：

收录情况： SCI

摘要： Tissue culture provides a useful system to investigate how plant hormones are involved in this process. Auxin and cytokinin are widely used in plant regeneration. Gibberellin is also an important plant hormone in regulating plant growth and development. It is interesting to know the effects of gibberellin and its signalling pathway on plant regeneration. In this report Arabidopsis thaliana landsberg (wild type), ga1-3 (gibberellin biosynthesis deficiency mutant), gai (gibberellin insensitive mutant), penta mutant (lacking GA1, GAI, RGA, RGL1, RGL2) and tetra mutant (lacking GAI, RGA, RGL1, RGL2) were used as materials to investigate how plant regeneration progress was affected in these mutants under different conditions. The results showed that more shoot buds were regenerated in ga1-3 and gai than in wild type, penta and tetra mutant in the normal shoot induction medium. The frequency of shoot bud regeneration in different mutants also varied remarkably when auxin : cytokinin ratio in the medium changed. Only penta mutant had shoot bud regeneration without auxin in the medium. When the ratio increased wild type, ga1-3 and gai had higher performance in shoot bud regeneration than penta mutant. When cytokinin level increased from 0.1 to 0.5 mg l(-1), shoot bud regeneration frequency of ga1-3, wild type and tetra increased remarkably except for that of penta and gai. Only the shoot bud regeneration frequency of gai did not change significantly when cytokinin level increased from 0.5 to 5 mg l(-1). These gibberellin-related mutants also responded differently to NPA (an auxin polar transport inhibitor) in plant regeneration. Our results indicate gibberellin and its related pathway are also involved in plant organogenesis: gibberellin inhibitor and auxin polar transport inhibitor can promote plant organogenesis. This might provide a new way for the regeneration of recalcitrant species.

分类号：

  • 相关文献

[1]Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision. Zhang, GQ,Zhang, DQ,Tang, GX,He, Y,Zhou, WJ. 2006

[2]Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family. Pearce, Stephen,Dubcovsky, Jorge,Huttly, Alison K.,Prosser, Ian M.,Li, Yi-Dan,Vaughan, Simon P.,Gallova, Barbora,Patil, Archana,Hedden, Peter,Phillips, Andrew L.,Li, Yi-Dan,Coghill, Jane A.,Dubcovsky, Jorge. 2015

[3]Molecular Cloning, Characterization, and Expression Analysis of Genes Encoding Gibberellin 20-Oxidase in Dasypyrum villosum Dwarf Mutant. Long, H.,Deng, G. B.,Pan, Z. F.,Yu, M. Q.,Cai, P.,Peng, Z. S..

[4]Fertility barriers in interspecific crosses within Viburnum. Xie, Wei-Jia,Wang, Ji-Hua,Leus, Leen,Van Laere, Katrijn.

[5]Identification of a novel gain-of-function mutant allele, slr1-d5, of rice DELLA protein. Zhang Yun-hui,Bian Xiao-feng,Zhang Suo-bing,Ling Jing,Wang Ying-jie,Fang Xian-wen,Wei Xiao-ying. 2016

[6]Tissue-specific expression and drought responsiveness of cell-wall invertase genes of rice at flowering. Ji, XM,Raveendran, M,Oane, R,Ismail, A,Lafitte, R,Bruskiewich, R,Cheng, SH,Bennett, J.

[7]Overexpression of soybean GmCBL1 enhances abiotic stress tolerance and promotes hypocotyl elongation in Arabidopsis. Li, Zhi-Yong,Xu, Zhao-Shi,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi,Li, Zhi-Yong,He, Guang-Yuan,Yang, Guang-Xiao.

[8]Gibberellin A(3) pretreatment increased antioxidative capacity of cucumber radicles and hypocotyls under suboptimal temperature. Li, Qingzhu,Li, Chaohan,Shi, Qinghua,Yu, Xianchang. 2011

[9]The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice. Qiao, Feng,Zhao, Kai-Jun,Qiao, Feng,Qiao, Feng,Zhao, Kai-Jun.

[10]A MADS-box gene NtSVP regulates pedicel elongation by directly suppressing a KNAT1-like KNOX gene NtBPL in tobacco (Nicotiana tabacum L.). Wang, Di,Chen, Xiaobo,Song, Gaoyuan,Kong, Xingchen,Geng, Shuaifeng,Yang, Jiayue,Wang, Bingnan,Wu, Liang,Li, Aili,Mao, Long,Zhang, Zenglin,Liu, Danmei.

[11]Molecular characterization and functional analysis of barley semi-dwarf mutant Riso no. 9265. Jia, Qiaojun,Zhang, Guoping,Jia, Qiaojun,Shang, Yi,Zhu, Jinghuan,Hua, Wei,Wang, Junmei,Yang, Jianming,Li, Chengdao. 2015

[12]Characterizations and molecular mapping of a novel dominant semi-dwarf gene Sdd(t) in rice (Oryza sativa). Liu, B. M.,Wu, Y. J.,Fu, X. D.,Qian, Q.. 2008

[13]EUI1, encoding a putative cytochrome P450 monooxygenase, regulates internode elongation by modulating gibberellin responses in rice. Luo, AD,Qian, Q,Yin, HF,Liu, XQ,Yin, CX,Lan, Y,Tang, JY,Tang, ZS,Cao, SY,Wang, XJ,Xia, K,Fu, XD,Luo, D,Chu, CC. 2006

[14]Comparative transcriptome analysis of berry-sizing effects of gibberellin (GA(3)) on seedless Vitis vinifera L.. Wang, Xicheng,Zhao, Mizhen,Wu, Weimin,Qian, Yaming,Wang, Zhuangwei,Korir, Nicholas Kibet.

[15]Cloning and characterization of HbJAZ1 from the laticifer cells in rubber tree (Hevea brasiliensis Muell. Arg.). Tian, W. -W.,Huang, W. -F.,Zhao, Y..

[16]Gibberellin stimulates regrowth after defoliation of sheepgrass (Leymus chinensis) by regulating expression of fructan-related genes. Cai, Yueyue,Liu, Gongshe,Chen, Shuangyan,Shao, Linhui,Li, Xiuqing.

[17]Gibberellins regulate the stem elongation rate without affecting the mature plant height of a quick development mutant of winter wheat (Triticum aestivum L.). Zhang, Ning,Xie, Yong-Dun,Guo, Hui Jun,Zhao, Lin-Shu,Xiong, Hong-Chun,Gu, Jia-Yu,Li, Jun-Hui,Zhao, Zi-Wei,Zhao, Shi-Rong,Liu, Lu-Xiang,Kong, Fu-Quan,Sui, Li.

[18]MechanismofSolanumrostratumDunalSeedGermination. 魏守辉,HongjuanHuang,ZhaofengHuang,JingchaoChen,JinyiChen,张朝贤. 2013

[19]Isolation and characterization of a GAI/RGA-like gene from Gossypium hirsutum. Liao, Wen-bin,Ruan, Meng-bin,Cui, Bai-ming,Lu, Jia-ju,Peng, Ming,Xu, Nan-fei.

[20]Autopolyploidy leads to rapid genomic changes in Arabidopsis thaliana. Liu, Shihong,Tian, Baoming,Wei, Fang.

作者其他论文 更多>>

微信小程序