Evaluation of elite inbred lines of maize (Zea mays L.) group Tangsipingtou for in vitro culture and plant regeneration

文献类型： 外文期刊

第一作者： Zhang, HW

作者： Zhang, HW;Tan, ZB;Chen, G;Li, JS

作者机构：

关键词： calli initiation;maize;plant regeneration;Tangsipingtou

期刊名称：MAYDICA （ 影响因子：0.542； 五年影响因子：0.605 ）

ISSN： 0025-6153

年卷期： 2004 年 49 卷 4 期

页码：

收录情况： SCI

摘要： Tangsipingtou is the most important maize heterotic group in china since 1970. Knowledge of its potential in in vitro culture and plant regeneration is limited. in the present work, fifteen Tangsipingtou elite inbred lines were tried in vitro to evaluate the influence of genotype, medium, plant hormones and culture protocols on embryo-derived callus induction and plant regeneration. The results showed that callus cultures could be induced from all tested inbreds. Plant regeneration potential was reduced to a certain extent along with culture aging. The modalities of callus induction and plant regeneration of different genotypes were different allowing to the hormone type and concentration. Among fifteen inbreds tested, seven were best on medium CIdcb (modified N6 medium with 3.3mg/L dicamba), and inbred Jing24 was best on CI24d1(modified N6 medium with 1mg/L 2,4-D) for callus induction. However, RH7, J7, HXZ853, ZH81 and HyJC were found with high performance of both callus induction and plant regeneration and were suitable genotypes for genetic transformation.

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