Effects of nuclease-treated fermentation product of Lactobacillus rhamnosus GCC-3 on growth, hepatic health and gut microbiota of zebrafish (Danio rerio) fed a high-fat diet

文献类型： 外文期刊

第一作者： Xie, Mingxu

作者： Xie, Mingxu;Li, Ming;Hao, Qiang;Zhou, Zhigang;Xie, Mingxu;Hao, Qiang;Olsen, Rolf Erik;Ringo, Einar;Yang, Yalin;Zhang, Zhen;Ran, Chao

作者机构：

关键词： Lactobacillus rhamnosus; Non-specific immunity; Liver health; Gut microbiota; Zebrafish

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 2352-5134

年卷期： 2023 年 29 卷

页码：

收录情况： SCI

摘要： Probiotics are reported to improve the nutrition, immunity, and health of fish. Nuclease can hydrolyze nucleic acids of probiotics to produce nucleotides. The present study investigated the effect of stabilized fermentation product of nuclease-treated Lactobacillus rhamnosus GCC-3 (GCC-3 NT) on growth, non-specific immunity, liver health, and gut microbiota of zebrafish (Danio rerio). Compared to the high-fat diet (HFD) group, GCC-3 NT did not affect the growth performance of zebrafish. However, GCC-3 NT treatment can significantly increase the lysozyme activity and the total antioxidant capacity of body surface mucus. In addition, dietary GCC-3 NT significantly reduced the content of hepatic triglycerides (TAG) in zebrafish while significantly increased the expression of acyl-coenzyme A oxidases 3 (ACOX3) and proliferator-activated receptor gamma coactivator 1 alpha (PGC1 alpha) compared with the HFD group. The 16S rRNA gene sequencing showed that GCC-3 NT reduced the relative abundance of Actinobacteria while increased Firmicutes at the phylum level. The relative abundance of Rhodococcus was significantly decreased and Lactobacillus and Staphylococcus abundance were significantly increased in the GCC-3 NT group compared to the HFD group. Furthermore, PCoA analysis showed GCC-3 NT diet had a significant effect on the autochthonous microbiota compared to the HFD diet. Together, our results showed that nuclease-treated L. rhamnosus fermentation product can improve the immunity, liver health and gut microbiota of zebrafish, suggesting that it can be potentially used as a functional feed additive for aquaculture.

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