Mouse hepatitis virus nucleocapsid protein interacts receptor for activated C kinase1, and regulates AKT signal pathway

文献类型： 外文期刊

第一作者： Zhang, Pengju

作者： Zhang, Pengju;Wu, Xinghong;Wang, Jun;Long, Miao

作者机构：

关键词： Hepatitis virus;nucleocapsid;receptor for activated C kinase 1;interaction

期刊名称：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE （ 影响因子：0.166； 五年影响因子：0.621 ）

ISSN： 1940-5901

年卷期： 2017 年 10 卷 3 期

页码：

收录情况： SCI

摘要： The nucleocapsid (N) protein of mouse hepatitis virus (MHV-N) has been reported to be a multifunctional protein involved in viral RNA replication and translation. However, how N protein interacts with host protein remains largely elusive. To identify cellular proteins that interact with the N protein, a cDNA library of mouse liver was screened using a yeast two-hybrid system assay. We have identified the receptor for activated C kinase 1 (RACK1) as a novel interaction partner of N protein by yeast two-hybrid system. The direct interaction and co-localization of N protein with RACK1 were confirmed by immunoprecipitation and confocal microscopy analysis, respectively. The mapping studies localized the critical N sequences for this interaction to amino acid 150-220 including SR motif by yeast two-hybrid system. Function assay showed that overexpressin N protein significantly inhibited RACK1 expression, thereby leading to suppress Akt pathway activation, which might contribute to induce apoptosis of host cells. To the best of our knowledge, this is the first report that MHV-N protein interacts with the RACK1 within host cells. These findings contribute to enhance our understanding of the pathological mechanisms of MHV.

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