Production of Bioactive Recombinant Bovine Chymosin in Tobacco Plants

文献类型： 外文期刊

第一作者： Wei, Zheng-Yi

作者： Wei, Zheng-Yi;Fan, Ming-Xia;Lin, Feng;Wei, Zheng-Yi;Zhang, Yu-Ying;Wang, Yun-Peng;Zhong, Xiao-Fang;Xing, Shao-Chen;Zhang, Yu-Ying;Xu, Nuo

作者机构：

关键词： chymosin;rennin;tobacco;milk coagulation;transformation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2016 年 17 卷 5 期

页码：

收录情况： SCI

摘要： Chymosin (also known as rennin) plays an essential role in the coagulation of milk in the cheese industry. Chymosin is traditionally extracted from the rumen of calves and is of high cost. Here, we present an alternative method to producing bovine chymosin from transgenic tobacco plants. The CYM gene, which encodes a preprochymosin from bovine, was introduced into the tobacco nuclear genome under control of the viral 35S cauliflower mosaic promoter. The integration and transcription of the foreign gene were confirmed with Southern blotting and reverse transcription PCR (RT-PCR) analyses, respectively. Immunoblotting analyses were performed to demonstrate expression of chymosin, and the expression level was quantified by enzyme-linked immunosorbent assay (ELISA). The results indicated recombinant bovine chymosin was successfully expressed at an average level of 83.5 ng/g fresh weight, which is 0.52% of the total soluble protein. The tobacco-derived chymosin exhibited similar native milk coagulation bioactivity as the commercial product extracted from bovine rumen.

分类号：

  • 相关文献

[1]Cloning and expression of a wild eggplant cytochrome P450 gene, StoCYP77A2, involved in plant resistance to Verticillium dahliae. Yang, Liu,Shi, Ce,Mu, Xiaoying,Liu, Chao,Shi, Ke,Zhu, Wenjiao,Yang, Qing,Yang, Liu.

[2]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[3]Variant Amino Acid Residues Alter the Enzyme Activity of Peanut Type 2 Diacylglycerol Acyltransferases. Zheng, Ling,Wan, Shubo,Peng, Zhenying,Zheng, Ling,Bian, Fei,Chen, Gao,Shan, Lei,Li, Xinguo,Wan, Shubo,Peng, Zhenying,Shockey, Jay. 2017

[4]Transformation of Liquidambar formosana L. via Agrobacterium tumefaciens using a mannose selection system and recovery of salt tolerant lines. Qiao, Guirong,Zhou, Jing,Jiang, Jing,Sun, Yuehua,Pan, Luanyin,Song, Honggai,Jiang, Jingmin,Zhuo, Renying,Sun, Yuehua,Wang, Xiaojuan,Sun, Zongxiu,Sun, Yuehua. 2010

[5]Progress in chloroplast genome analysis. Liu, Clarke Jihong,Xing Shao-Chen. 2008

[6]Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae. Rehman, Latifur,Su, Xiaofeng,Guo, Huiming,Qi, Xiliang,Cheng, Hongmei. 2016

[7]Regeneration of Chinese cabbage transgenic plants expressing antibacterial peptide gene and cowpea trypsin inhibitor gene. Zhao, Junliang,Liang, Aihua,Zhu, Zhen,Tang, Yixiong. 2006

[8]Transformation of sulfate and organic S in rice straw in flooded paddy soils and its availability to plants using sulfur-35 labeling. Zhou, W,Li, ST,He, P,Lin, B. 2006

[9]A new time-saving transformation system for Brassica napus. Kong, Fanming,Li, Juan,Tan, Xiaoli,Zhang, Lili,Zhang, Zhiyan,Ma, Xiaoke,Qi, Cunkou. 2009

[10]Transient beta-glucuronidase expression in asparagus protoplasts following electroporation. Chen, GY,Conner, AJ,Fautrier, AG,Field, RJ. 1999

[11]Enhancement of salt tolerance in alfalfa transformed with the gene encoding for betaine aldehyde dehydrogenase. Liu, Zi-Hui,Zhang, Hong-Mei,Li, Guo-Liang,Guo, Xiu-Lin,Zhang, Yan-Min,Liu, Zi-Hui,Zhang, Hong-Mei,Li, Guo-Liang,Guo, Xiu-Lin,Zhang, Yan-Min,Chen, Shou-Yi,Liu, Gui-Bo. 2011

[12]An efficient protocol for the production of chit42 transgenic Furenzhi banana (Musa spp. AA group) resistant to Fusarium oxysporum. Hu, Chun-Hua,Wei, Yue-Rong,Huang, Yong-Hong,Yi, Gan-Jun,Hu, Chun-Hua,Wei, Yue-Rong,Huang, Yong-Hong,Yi, Gan-Jun. 2013

[13]A callus transformation system for gene functional studies in soybean. Xu Kun,Zhang Xiao-mei,Fan Cheng-ming,Chen Fu-lu,Zhu Jin-long,Fu Yong-fu,Zhang Shi-long,Chen Qing-shan. 2017

[14]Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Jones, H. D.,Li, K. X.,He, Y.,Chen, S.,Chen, X. M.,Wang, D. S.,Xia, L. Q.,Wang, D. W.. 2010

[15]Gene transfer strategy for kiwifruit to obtain pure transgenic plant through inducing adventitious roots in leaf explants with Agrobacterium tumefaciens. Li, M,Huang, ZG,Han, LX,Zhao, GR,Li, YH. 2003

[16]Research Progress on Transformation Maize Mediated by Agrobacterithm Tumefaciens. Li, Xiuping,Li, Xiuping,Jiang, Lijing,Liu, Na. 2011

[17]Agrobacterium tumefaciens-mediated transformation of poinsettia, Euphorbia pulcherrima, with virus-derived hairpin RNA constructs confers resistance to Poinsettia mosaic virus. Clarke, Jihong Liu,Spetz, Carl,Haugslien, Sissel,Xing, Shaochen,Dees, Merete W.,Blystad, Dag-Ragnar,Xing, Shaochen,Dees, Merete W.,Moe, Roar. 2008

[18]Transformation of deoxynivalenol and its acetylated derivatives in Chinese steamed bread making, as affected by pH, yeast, and steaming time. Wang, Bujun.

[19]ELEVATED ENDOGENOUS ISOPENTENYL ADENINE CONTENT IS CORRELATED WITH AN EXTREMELY SHOOTY RICE PHENOTYPE. ZHU, YX,QIN, RZ,SHAN, XY,CHEN, ZL.

[20]Screening and identification of insertion mutants from Bipolaris eleusines by mutagenesis based on restriction enzyme-mediated integration. Zhang Jianping,Duan Guifang,Zhu Kai,Zhou Yongjun,Lu Yongliang,Yu Liuqing.

作者其他论文 更多>>

微信小程序