A structural variation in the promoter of the leucoanthocyanidin reductase gene AaLAR1 enhances freezing tolerance by modulating proanthocyanidin accumulation in kiwifruit (Actinidia arguta)

文献类型： 外文期刊

第一作者： Sun, Shihang

作者： Sun, Shihang;Qi, Xiujuan;Zhang, Zhenzhen;Sun, Leiming;Wang, Ran;Li, Yukuo;Chen, Jinyong;Gu, Hong;Fang, Jinbao;Lin, Miaomiao;Sun, Shihang;Qi, Xiujuan;Li, Yukuo;Chen, Jinyong;Fang, Jinbao;Lin, Miaomiao

作者机构：

关键词： Actinidia chinensis; freezing tolerance; LAR1; promoter variation; transcription factor

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.0； 五年影响因子：7.6 ）

ISSN： 0140-7791

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Proanthocyanidins (PAs) are important metabolites that enhance freezing tolerance of plants. Actinidia arguta, especially freezing-tolerant germplasms, accumulate abundant PAs in dormant shoots and thereby enhance freezing tolerance, but the underlying mechanism is unknown. In this study, we used two A. arguta with contrasting cold-resistant phenotypes, KL and RB, to explore the mechanisms in response to cold tolerance. We determined that a leucoanthocyanidin reductase gene (AaLAR1) was more highly expressed in freezing-tolerant KL than in freezing-sensitive RB. Moreover, overexpressing AaLAR1 in kiwifruit promoted PAs biosynthesis and enhanced cold tolerance. The AaLAR1 promoters of various A. arguta germplasms differ due to the presence of a 60-bp deletion in cold-tolerant genotypes that forms a functional binding site for MYC-type transcription factor. Yeast one-hybrid and two-hybrid, dual-luciferase reporter, bimolecular fluorescence complementation and coimmunoprecipitation assays indicated that the AaMYC2a binds to the MYC-core cis-element in the AaLAR1 promoter with the assistance of AaMYB5a, thereby promoting PAs accumulation in the shoots of cold-tolerant kiwifruit. We conclude that the variation in the AaLAR1 promoter and the AaMYC2a-AaMYB5a-AaLAR1 module shape freezing tolerance in A. arguta. The identification of a key structural variation in the AaLAR1 promoter offers a new target for resistance breeding of kiwifruit.

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