Isolation and analysis of differentially expressed genes from peanut in response to challenge with Ralstonia solanacearum

文献类型： 外文期刊

第一作者： Ding, Yu Fei

作者： Ding, Yu Fei;Wang, Chuan Tang;Tang, Yue Yi;Wang, Xiu Zhen;Wu, Qi;Yu, Hong Tao;Zhang, Jian Cheng;Cui, Feng Gao;Song, Guo Sheng;Yu, Shan Lin;Hu, Dong Qing;Gao, Hua Yuan

作者机构：

关键词： Arachis hypogaea;ARF;cyclophilin;differentially expressed genes;genefishing;Ralstonia solanacearum

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2012 年 15 卷 5 期

页码：

收录情况： SCI

摘要： Background: Bacterial wilt caused by Ralstonia solanacearum is the most devastating disease in peanut. Planting resistant peanut cultivars is deemed as the sole economically viable means for effective control of the disease. To understand the molecular mechanism underlying resistance and facilitate breeding process, differences in gene expression between seeds of Rihua 1 (a Virginia type peanut variety resistant to bacterial wilt) inoculated with the bacterial pathogen suspension (10(9) cfu ml(-1)) and seeds of the same cultivar treated with water (control), were studied using the Genefishing (TM) technology. Results: A total of 25 differentially expressed genes were isolated. Expression of genes encoding cyclophilin and ADP- ribosylation factor, respectively, were further studied by real time RTPCR, and full length cDNAs of both genes were obtained by rapid amplification of cDNA ends. Conclusions: The study provided candidate genes potentially useful for breeding peanut cultivars with both high yield and bacterial wilt resistance, although confirmation of their functions through transgenic studies is still needed.

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