Cloning and identification of a novel C18-Delta 9 polyunsaturated fatty acid specific elongase gene from DHA-producing Isochrysis galbana H29

文献类型： 外文期刊

第一作者： Ou, Xiuyuan

作者： Ou, Xiuyuan;Xing, Laijun;Li, Mingchun;Li, Ming;Ou, Xiuyuan;Xing, Laijun;Li, Mingchun;Li, Ming;Yang, Xiangdong;Guo, Dongquan;Qian, Xueyan

作者机构：

关键词： Delta 9-elongase;polyunsaturated fatty acids;Isochrysis galbana;arachidonic acid;eicosapentaenoic acid

期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）

ISSN： 1226-8372

年卷期： 2012 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Isochrysis galbana, a marine prymnesiophyte microalga, is able to produce a high level of long chain polyunsaturated fatty acids such as docosahexaenoic acid (DHA, C22:6n-3). In this article, a novel gene (IgASE2) that encoded a C18-Delta 9 polyunsaturase fatty acids specific (C18-Delta 9-PUFAs-specific) elongase was isolated and characterized from DHA-rich microalga, I. galbana H29. A full-length cDNA of 1653 bp was cloned by rapidamplification of cDNA ends (RACE) PCR techniques. The IgASE2 contained a 786 bp ORF encoding a protein of 261 amino acids that shared 87% identity with the reported Delta 9-elongase IgASE1, a 44 bp 5' untranslated region and an 823 bp 3' untranslated region. The function of IgASE2 was demonstrated by its heterologous expression in Saccharomyces cerevisiae. In S. cerevisiae, IgASE2 elongated linoleic acid (LA, C18:2n-6), alpha-linolenic (ALA, C18:3n-3) to eicosadienoic acid (EDA, C20:2n-6) and eicosatrienoic acid (ETrA, C20:3n-3). The conversion ratios of LA to EDA and ALA to ETrA were 60.47 and 58.36%, respectively. However, IgASE2 could not catalyze the elongation reactions of oleic acid (OA, C18:1n-9) and other fatty acids. These results confirmed that IgASE2 had C18-Delta 9-PUFAs-specific elongase activity.

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