Transformation of alfalfa chloroplasts and expression of green fluorescent protein in a forage crop

文献类型： 外文期刊

第一作者： Wei, Zhengyi

作者： Wei, Zhengyi;Liu, Yanzhi;Lin, Chunjing;Wang, Yunpeng;Cai, Qin'an;Dong, Yingshan;Xing, Shaochen

作者机构：

关键词： Alfalfa;Chloroplast transformation;Green fluorescent protein;Medicago sativa L.

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2011 年 33 卷 12 期

页码：

收录情况： SCI

摘要： The ability to transform chloroplasts in multiple species is important for improving agricultural traits. Chloroplast transformation of alfalfa (Medicago sativa L.), a useful forage plant with high market value, was achieved using a vector carrying aadA and gfp genes being introduced into the chloroplasts of alfalfa via particle bombardment using leaves and calli as explants. Resistant somatic embryos were generated and developed into plantlets from explants. The transformation efficiency was 1.3% for callus explants and 2.7% for leaf explants. PCR and Southern blotting analyses revealed that the foreign genes were integrated into the transformed chloroplast genome. The occurrence of GFP was further confirmed by fluorescence microscopy. Expression of foreign genes in alfalfa chloroplasts is therefore possible, and provides a novel means for genetic improvement of agronomically important traits and production of value-added proteins.

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