Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution

文献类型： 外文期刊

第一作者： He, Li

作者： He, Li;Zhao, Hainan;Zhang, Tao;Jiang, Jiming;Braz, Guilherme T.;Torres, Giovana A.;He, Li;Zhang, Tao;Semrau, Kassandra;Rouillard, Jean-Marie;Rouillard, Jean-Marie;Jiang, Jiming;Jiang, Jiming

作者机构：

关键词： chromosome identification;karyotype;oligo-FISH;chromosome painting;translocation

期刊名称：GENETICS （ 影响因子：4.562； 五年影响因子：4.845 ）

ISSN： 0016-6731

年卷期： 2018 年 208 卷 2 期

页码：

收录情况： SCI

摘要： Development of a eukaryotic karyotype relies on identification of individual chromosomes in the species, which has been accomplished only in a limited... Developing the karyotype of a eukaryotic species relies on identification of individual chromosomes, which has been a major challenge for most nonmodel plant and animal species. We developed a novel chromosome identification system by selecting and labeling oligonucleotides (oligos) located in specific regions on every chromosome. We selected a set of 54,672 oligos (45 nt) based on single copy DNA sequences in the potato genome. These oligos generated 26 distinct FISH signals that can be used as a bar code or banding pattern to uniquely label each of the 12 chromosomes from both diploid and polyploid (4x and 6x) potato species. Remarkably, the same bar code can be used to identify the 12 homeologous chromosomes among distantly related Solanum species, including tomato and eggplant. Accurate karyotypes based on individually identified chromosomes were established in six Solanum species that have diverged for >15 MY. These six species have maintained a similar karyotype; however, modifications to the FISH signal bar code led to the discovery of two reciprocal chromosomal translocations in Solanum etuberosum and S. caripense. We also validated these translocations by oligo-based chromosome painting. We demonstrate that the oligo-based FISH techniques are powerful new tools for chromosome identification and karyotyping research, especially for nonmodel plant species.

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