Intra- and inter-isolate variation of ribosomal and protein-coding genes in Pleurotus: implications for molecular identification and phylogeny on fungal groups

文献类型： 外文期刊

第一作者： Li, Qian

作者： Li, Qian;Peng, Wei-Hong;Zhou, Jie;Cao, Xue-Lian;Wang, Di;Huang, Zhong-Qian;Tan, Wei;Gan, Bing-Cheng;Li, Qian;Li, Yu;Li, Qian

作者机构：

关键词： Edible mushroom;Intra-isolate polymorphism;Specific variation

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: The internal transcribed spacer (ITS), RNA polymerase II second largest subunit (RPB2), and elongation factor 1-alpha (EF1 alpha) are often used in fungal taxonomy and phylogenetic analysis. As we know, an ideal molecular marker used in molecular identification and phylogenetic studies is homogeneous within species, and interspecific variation exceeds intraspecific variation. However, during our process of performing ITS, RPB2, and EF1a sequencing on the Pleurotus spp., we found that intra-isolate sequence polymorphism might be present in these genes because direct sequencing of PCR products failed in some isolates. Therefore, we detected intra-and inter-isolate variation of the three genes in Pleurotus by polymerase chain reaction amplification and cloning in this study. Results: Results showed that intra-isolate variation of ITS was not uncommon but the polymorphic level in each isolate was relatively low in Pleurotus; intra-isolate variations of EF1a and RPB2 sequences were present in an unexpectedly high amount. The polymorphism level differed significantly between ITS, RPB2, and EF1a in the same individual, and the intra-isolate heterogeneity level of each gene varied between isolates within the same species. Intra-isolate and intraspecific variation of ITS in the tested isolates was less than interspecific variation, and intra-isolate and intraspecific variation of RPB2 was probably equal with interspecific divergence. Meanwhile, intra-isolate and intraspecific variation of EF1a could exceed interspecific divergence. These findings suggested that RPB2 and EF1a are not desirable barcoding candidates for Pleurotus. We also discussed the reason why rDNA and protein-coding genes showed variants within a single isolate in Pleurotus, but must be addressed in further research. Conclusions: Our study demonstrated that intra-isolate variation of ribosomal and protein-coding genes are likely widespread in fungi. This has implications for studies on fungal evolution, taxonomy, phylogenetics, and population genetics. More extensive sampling of these genes and other candidates will be required to ensure reliability as phylogenetic markers and DNA barcodes.

分类号：

  • 相关文献

[1]White mold on cultivated morels caused by Paecilomyces penicillatus. Peng, Wei-Hong,Miao, Ren-Yun,Tang, Jie,Chen, Ying,Liu, Li-Xu,Wang, Di,Gan, Bing-Cheng,He, Xiao-Lan,Peng, Wei-Hong,Miao, Ren-Yun,Chen, Ying,Liu, Li-Xu,Wang, Di,Gan, Bing-Cheng. 2017

[2]Purification of a novel hydrophobin PN1 involved in antibacterial activity from an edible mushroom Pleurotus nebrodensis. Zhang, Rui Ying,Gu, Jin Gang,Zhang, Jin Xia,Hu, Qing Xiu,Zhang, Rui Ying,Gu, Jin Gang,Zhang, Jin Xia,Hu, Qing Xiu,Hu, Dan Dan,Goodwin, Paul H..

[3]What is the radicate Oudemansiella cultivated in China?. Hao, Yan-Jia,Zhao, Qi,Yang, Zhu L.,Hao, Yan-Jia,Wang, Shou-Xian.

[4]A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea. Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Hu, D. D.,Zhang, G. Q.,Wang, H. X.,Zhang, R. Y.,Ng, T. B.. 2011

[5]Purification and characterization of a novel metalloprotease from fruiting bodies of Oudemansiella radicata. Geng, Xueran,Geng, Xueran,Te, Rigen,Wang, Hexiang,Geng, Xueran,Te, Rigen,Wang, Hexiang,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun. 2017

[6]Effect of Supplements Mn2+, Cu2+, and Aromatic Compounds and Penicillium decumbens on Lignocellulosic Enzyme Activity and Productivity of Catathelasma ventricosum. Sun, Jun,Yang, Yanjun,Liu Yuntao,Sun, Jun,Su, Yujie,Yang, Yanjun,Luo, Zeyu,Rao, Shengqi. 2013

[7]Anoxia and anaerobic respiration are involved in "spawn-burning" syndrome for edible mushroom Pleurotus eryngii grown at high temperatures. Zhang, Rui Ying,Zhang, Yong Yue,Huang, Chen Yang,Chen, Qiang,Gao, Wei,Wu, Xiang Li,Zou, Ya Jie,Qu, Ji Bin,Zhang, Jin Xia,Zhang, Rui Ying,Zhang, Yong Yue,Huang, Chen Yang,Chen, Qiang,Gao, Wei,Wu, Xiang Li,Zou, Ya Jie,Qu, Ji Bin,Zhang, Jin Xia,Hu, Dan Dan,Goodwin, Paul H..

[8]Development of AFLP markers and phylogenetic analysis in Hypsizygus marmoreus. Wang, Lin,Hu, Xiaohua,Feng, Zhiyong,Pan, Yingjie.

[9]Use of intersimple sequence repeats markers to develop strain-specific SCAR markers for Lentinula edodes. Qin, LH,Tan, Q,Chen, MJ,Pan, YJ.

作者其他论文 更多>>

微信小程序