Preparation, purification and regeneration optimizing research of protoplasts from Rhizoctonia solani

文献类型： 外文期刊

第一作者： Sun, Zhiguang

作者： Sun, Zhiguang;Qin, Peigang;Tang, Changqing;Fu, Rongtao;Liu, Yao;Li, Ping;Zheng, Aiping;Li, HaoJie;Feng, Haitao;Sun, Zhiguang;Qin, Peigang;Tang, Changqing;Fu, Rongtao;Liu, Yao;Li, Ping;Zheng, Aiping

作者机构：

关键词： Preparation;protoplast;purification;regeneration;Rhizoctonia solani

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2012 年 6 卷 13 期

页码：

收录情况： SCI

摘要： The aim of this work was to study the standardization of conditions to obtain and regenerate protoplasts of Rhizoctonia solani. The optimal way of harvesting 1.67 x 10(9) protoplasts per gram from Rhizoctonia solani was to deal with 12 h aged mycelium in 0.6 M MgSO4 stabilizer (pH 5.2) combining cellulase and driselase at 35 degrees C for 3 h. Meanwhile, the most suitable condition for protoplast regeneration had been attained. The condition was to deal with 16 h aged mycelium in 0.6 M MgSO4 stabilizer (pH 5.2) combining cellulase with driselase at 35 degrees C for 2.5 h, and incubation at 26 degrees C in the regeneration medium. Furthermore, a two-phase system of 0.6 M sucrose and 0.6 M mannitol was developed for separation and purification of intact protoplasts from broken protoplasts, excellent results were obtained. There was a breakthrough for protoplast technology of R. solani and other fungus was recorded in this paper.

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