ITS sequence-based identification and utilization evaluation of "Nanjiang" (Lonicera similis Hemsl.), a local cultivar in Sichuan, China

文献类型： 外文期刊

第一作者： Chen, Xiaomin

作者： Chen, Xiaomin;Bai, Linhan;Hu, Shangqin;Huang, Lulin;Yang, Xiao;Tong, Wen

作者机构：

关键词： Flos Lonicerae;Lonicera similis Hemsl.;Identification;Antibacterial activity;Chlorogenic acid

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN： 0925-9864

年卷期： 2012 年 59 卷 4 期

页码：

收录情况： SCI

摘要： The internal transcribed spacer (ITS) region was employed to analyze phylogeny of 20 populations, including the local cultivar "Nanjiang", belonging to 7 species of Lonicera L. 20 ITS sequences that we obtained range from 614 to 618 bp, and the G+C content was higher than 60% with the average value 63.5%. The sequences of 5.8S nrDNA was highly conservative in both length and component, while ITS2 had more variation than ITS1 in sequence component and length. For the ITS complete sequence, the mutation and information sites were 139 and 117 with the content of 22.38 and 18.84%, respectively. The dendrogram analysed by neighbor-joining method showed that "Nanjiang" and Lonicera similis Hemsl. comprised a same cluster with a higher bootstrap support, indicating "Nanjiang" would be a variation or subspecies of L. similis Hemsl. The results of antibacterial activity test and active compounds identification showed that "Nanjiang" had the best antibacterial activity and the highest yield of chlorogenic acid, the active component of Flos Lonicerae, in the six species of Lonicera L.

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