Construction and Chromatographic Performance of a Novel Triazole Bridged Hybrid Bilayer Cyclodextrin Chiral Stationary Phase

文献类型： 外文期刊

第一作者： Zhang Lifang

作者： Zhang Lifang;Wang Yong;Zhang Lifang;Wang Yong;Zhao Jie

作者机构：

关键词： click chemistry;bilayer cyclodextrin;chiral stationary phase;enantiorecognition;HPLC ester

期刊名称：ACTA CHIMICA SINICA （ 影响因子：2.668； 五年影响因子：1.839 ）

ISSN： 0567-7351

年卷期： 2015 年 73 卷 11 期

页码：

收录情况： SCI

摘要： A novel tandem-inverted triazole-bridged duplex "native-acetylated" hybrid cyclodextrin (CD) stationary phase material (ANCDCSP) was constructed via a surface-up 'click' approach. Mono-6-azido-CD was first immobilized onto the pre-dried silica surfaces as the down layer via ether linkage on C2 position, followed by acetylation of the cyclodextrin OH groups with acetic anhydride. The top layer was fabricated by anchoring the synthesized alkyne functionalized CD onto the down CD layer via organic soluble Cu(I) catalytic 1,3-dipolar cycloaddition reaction (click chemistry) reported by us previously. The obtained crude material was purified by washing with N,N-dimethylformamide (DMF) twice followed by Soxlet extraction with acetone to afford the novel triazole-bridged duplex hybrid CD CSP and its structure was characterized via Fourier Transfer Infrared Spectroscopy (FTIR), thermogravimetric analysis (TG) and elemental analysis (EA). The hybrid bilayer ANCDCSP can provide multiple interaction sites such as H-bonding (OH, C=O, NH), steric effects, dipole-dipole and synergistic effect inclusion complexation, which helps to broaden the CSP's enantioselectivity profile and enhance the enantioselectivity to some specific analytes. The CSP was applied for HPLC enantioseparation of various chiral compounds such as dansyl amino acids, carboxylic aryl acids, isoxazolines and some other racemates under reversed-phase separation mode using methanol/water and methanol/buffer as the mobile phases. Most of the dansyl amino acids and carboxylic aryl acids as well as bendroflumethiazide can be baseline separated. The isoxazolines, phenyl oxirane, atropine, DL-norleucine methyl ester and benfluorex were partially separated. The resolutions of Dns-Phe, DL-3PhLacA and Bendroflumethiazide reached 5.3, 4.1 and 4.1, respectively. The separation ability of the current CSP is superior to that of the duplex native cyclodextrins chiral stationary phase (DCDCSP) prepared by our group previously, especially in separation of dansyl amino acids, isoxazolines and bendroflumethiazide. Besides, the current CD CSP affords relatively good stability, which was verified by the satisfied reproducibility after 150 runs with or without buffer (pH 4.10).

分类号：

  • 相关文献

[1]Enantioseparation Performance Tuning of Novel Cyclodextrin Chiral Separation Materials via Click Chemistry. Wu Zhi-Hua,Li Shen,Wang Yong,Zhao Jie,Wang Yong. 2016

[2]Quantification of DNA through a fluorescence biosensor based on click chemistry. Yue, Guiyin,Huang, Xijing,Ye, Wenmei,Qiu, Bin,Lin, Zhenyu,Chen, Guonan,Qiu, Suyan,Ye, Huazhen.

[3]Preparation of a novel carboxyl stationary phase by "thiol-ene" click chemistry for hydrophilic interaction chromatography. Peng, Xi-Tian,Liu, Tao,Ji, Shu-Xian,Feng, Yu-Qi,Peng, Xi-Tian.

[4]Direct enantioseparation of nitrogen-heterocyclic pesticides on amylose-tris-(5-chloro-2-methylphenylcarbamate) by reversed-phase high-performance liquid chromatography. Yang, Wenwen,Qiu, Jing,Chen, Tianjin,Yang, Shuming,Yang, Wenwen,Qiu, Jing,Chen, Tianjin,Yang, Shuming,Yang, Wenwen,Hou, Shicong.

[5]Green synthesis of a typical chiral stationary phase of cellulose-tris(3,5-dimethylphenylcarbamate). Bai, Lian-yang,Liu, Run-Qiang,Zhang, Yi-Jun,Zhang, Yu-Ping,Bai, Lian-yang. 2013

[6]Direct Enantioseparation of Nitrogen-Heterocyclic Pesticides on Cellulose-Based Chiral Column by High-Performance Liquid Chromatography. Chai, Tingting,Yang, Wenwen,Hou, Shicong,Chai, Tingting,Yang, Wenwen,Qiu, Jing.

[7]Comparison of Enantioseparations of 19 Chiral Polychlorinated Biphenyls by 5 Different Polysaccharides Chiral Columns. Xu Na-Na,Mu Peng-Qian,Jia Qi,Chai Ting-Ting,Yin Zhi-Qiang,Shu-Ming, Yang,Jing, Qiu,Chai Ting-Ting. 2015

[8]Synthesis of Cellulose-2,3-bis(3,5-dimethylphenylcarbamate) in an Ionic Liquid and Its Chiral Separation Efficiency as Stationary Phase. Bai, Lianyang,Liu, Runqiang,Zhang, Yijun,Huang, Mingxian,Chen, Jun,Zhang, Yuping,Bai, Lianyang. 2014

作者其他论文 更多>>

微信小程序