Application of Atractylodes Macrocephala Koidz Extract in Methicillin - Resistant Staphylococcus Aureus

文献类型： 外文期刊

第一作者： Xu, Zhenguo

作者： Xu, Zhenguo;Cai, Yuhua;Fan, Gaofu;Liu, Xiushu;Dai, Yin

作者机构：

关键词： Atractylodes macrocephala Koidz;Atractylodes macrocephala Koidz extractive;MRSA;Mice;Models

期刊名称：13TH GLOBAL CONGRESS ON MANUFACTURING AND MANAGEMENT

ISSN： 1877-7058

年卷期： 2017 年 174 卷

页码：

收录情况： SCI

摘要： To study the effect of Atractylodes macrocephala Koidz extractive on MRSA. First we used hydrogen peroxide aqueous to oxygenize the sample, and then added into ascorbic acid which had protected the oxidative product to extract effective consituent of Actractylodes macrocephala Koidz, measuring its antibacterial activity with oxford cup method and the minimum inhibitory concentration with double dilution method. Next, we divided mice with Otisis Media into six groups: high dose of Atractylodes group, medium dose of Atractylodes group, low dose of Actractylodes group, Oxfoxacin Ear Drops group, Vancomycin group and blank model group. The extracting method of oxidizing before preserving oxidative product leads to 9.5% of Actractylodes macrocephala Koidz extractive, with a minimum inhibitory concentration of 0.781 mg/ml. Comparing with the blank model group, the number of bacterial colonies had sharply declined after 1 day of medication. On the second day of medication treatment, in the groups with Atractylodes in various concentration, an obvious decline of bacterial colonies were also observed. Although the effect of Atractylodes had came slower than Oxfoxacin and Vancomycin, but it was proved to be effective to MRSA within animal bodies. Besides, there were differences among various concentration of Atractylodes, higher concentration tended to have better antibacterial function. The method of extracting Atractylodes in this research was easy and productive. Through experiments in vivo and vitro, it proved that it could be independently used in MRSA, and we could speculate that the effect of Atractylodes macrocephala Koidz extractive was concentration-based. (C) 2016 The Authors. Published by Elsevier Ltd.

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