Simple and rapid detection of Tilletia horrida causing rice kernel smut in rice seeds

文献类型： 外文期刊

第一作者： Chen, Yu

作者： Chen, Yu;Yang, Xue;Kyaw, Ei Phyu;Zhang, Ai-Fang;Gu, Chun-Yan;Zang, Hao-Yu;Gao, Tong-Chun;Chen, Yu;Yang, Xue;Kyaw, Ei Phyu;Zhang, Ai-Fang;Gu, Chun-Yan;Zang, Hao-Yu;Gao, Tong-Chun;Chen, Yu;Yang, Xue;Kyaw, Ei Phyu;Zhang, Ai-Fang;Gu, Chun-Yan;Zang, Hao-Yu;Gao, Tong-Chun;Yao, Jian;Li, Yun-Fei;Kyaw, Ei Phyu

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： A simple and rapid method for the detection of Tilletia horrida, the causal agent of rice kernel smut, in rice seeds is developed based on specific polymerase chain reaction (PCR). To design the specific primers for the detection of T. horrida, partial sequences of internal transcribed spacer (ITS) DNA region of T. horrida, T. controversa, T. walkeri, T. ehrhartae, T. indica and T. caries were analyzed and compared. A 503-bp fragment was amplified with the designed primers from the T. horrida genomic DNA. However, no PCR product was obtained from the DNA of other five Tilletia species and 22 fungal plant pathogens tested in the present work indicating the specificity of the primers for the detection of T. horrida. The PCR was performed by directly using the spores, isolated from the 21 different rice seed samples, as template DNA. The T. horrida was detected in 6 of the samples, indicating that 28.6% of the rice samples were contaminated with the kernel smut pathogen. This simple PCR based diagnostic assay can be applied for the direct and rapid detection and identification of T. horrida to screen large numbers of rice seed samples.

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