Single Nucleotide Polymorphism Scanning and Expression of the Pig PPARGC1A Gene in Different Breeds
文献类型: 外文期刊
第一作者: Li, Qinggang
作者: Li, Qinggang;Lu, Yunfeng
作者机构:
关键词: Pig;Lipids;PPARGC1A;Gene expression;SNPs
期刊名称:LIPIDS ( 影响因子:1.88; 五年影响因子:2.417 )
ISSN: 0024-4201
年卷期: 2014 年 49 卷 10 期
页码:
收录情况: SCI
摘要: Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a candidate gene for lean meat production because it plays a key role in lipid metabolism. In this study, SNPs within the porcine PPARGC1A gene were investigated using PCR-sequencing and PCR-RFLP. Quantitative real-time PCR and Western blot were then used to analyze mRNA and protein expression in longissimus dorsi muscle (LM), liver, and backfat tissues of Dianna small-ear pigs (DSP, n = 6), Tibetan pigs (TP, n = 6), and large white pigs (LW, n = 6). Five novel SNPs (g.-1269A > G in the 5'-upstream regulatory region; g.190C > T, g.218C > A and g.234C > A in exon 8; and g.20C > T in intron 10) and three previously identified SNPs (g.417A > T in exon 8; g.56C > A in exon 9; and g.34G > A in intron 9) were found. Of these, only two, g.-1269A > G and g.234C > A, had three different genotypes in the three breeds (DSP, n = 63; TP, n = 51; and LW, n = 52). Expression was highest in LM, modest in the liver, and minimal in backfat. In LM tissue, LW had higher mRNA and protein levels than DSP and TP (P < 0.05), and there was a negative correlation between gene expression and intramuscular fat (IMF) content. LW had numerically higher expression in liver and backfat tissues than DSP and TP, and the differences in protein levels were significant (P < 0.05 in liver, P < 0.01 in backfat). In conclusion, PPARGC1A may play a key role in down-regulating lipid deposition, and the SNPs with differential genotype distribution among the three pig breeds may be related to gene expression and fat deposition.
分类号:
- 相关文献
作者其他论文 更多>>
-
Effect of Nanoselenium and Ferment Application on the Rhizosphere Soil of Kiwifruit
作者:Liu, Yanan;He, Qiqi;Lu, Yunfeng;Liu, Yanan;Zhang, Sipu;Niu, Jiajia;Xu, Zhifei;Yang, Yongfeng
关键词:Kiwifruit; Nanoselenium; Ferment; Soil microbial community; Soil enzyme activities
-
The effect of nano-selenium application on the quality and flavor of kiwifruit
作者:Liu, Yanan;Lu, Yunfeng;Liu, Yanan;Zhang, Sipu;Niu, Jiajia;Xu, Zhifei;Yang, Yongfeng
关键词:Kiwifruit; Nano-selenium; Amino acids; Antioxidant capacity; Flavouring substance
-
Nanoselenium foliar application boosts antioxidant capacity and soluble solids in kiwifruit
作者:Liu, Yanan;Hu, Yuanzheng;Lu, Yunfeng;Zhang, Sipu;Niu, Jiajia;Xu, Zhifei;Yang, Yongfeng
关键词:Kiwifruit; Nanoselenium; Antioxidant capacity; Soluble solids content; Genomics
-
Identification of candidate genes that specifically regulate subcutaneous and intramuscular fat deposition using transcriptomic and proteomic profiles in Dingyuan pigs
作者:Zhang, Pan;Zhang, Yawen;Zhang, Bo;Zhang, Hao;Li, Qinggang;Wu, Yijing
关键词:
-
Candidate gene screening for lipid deposition using combined transcriptomic and proteomic data from Nanyang black pigs
作者:Wang, Liyuan;Lu, Yunfeng;Wang, Liyuan;Zhang, Yawen;Zhang, Bo;Zhong, Haian;Zhang, Hao;Wang, Liyuan
关键词:Genetic divergence; Lipid deposition; Multi-omics; Nanyang black pig; Phenotypic divergence; Proteome; Transcriptome
-
Functional analysis of the brain natriuretic peptide gene for high-altitude adaptation in Tibetan pigs
作者:Tian, Xiaolong;Ma, Jun;Zhang, Pan;Zhang, Hao;Wu, Changxin;Wu, Yijing;Li, Qinggang
关键词:BNP; High-altitude adaptation; Gene expression; SNP; Tibetan Pig
-
Identification of differentially expressed genes in longissimus dorsi muscle between Wei and Yorkshire pigs using RNA sequencing
作者:Xu, Jingen;Jin, Erhui;Gu, Youfang;Li, Shenghe;Xu, Jingen;Wang, Chonglong;Li, Qinggang
关键词:Transcriptome sequencing; Pig; Meat quality; Breed; Differentially expressed genes; Intramuscular fat