Role of serotonin in the intestinal mucosal epithelium barrier in weaning mice undergoing stress-induced diarrhea

文献类型： 外文期刊

第一作者： Dong, Yulan

作者： Dong, Yulan;Wang, Zixu;Cao, Jing;Chen, Yaoxing;Qin, Zhuoming

作者机构：

关键词： Serotonin;Stress-induced diarrhea;Intestine;Tight junction

期刊名称：JOURNAL OF MOLECULAR HISTOLOGY （ 影响因子：2.611； 五年影响因子：2.502 ）

ISSN： 1567-2379

年卷期： 2018 年 49 卷 1 期

页码：

收录情况： SCI

摘要： Stress-induced diarrhea is a frequent and challenging threat to humans and domestic animals. Serotonin (5-HT) has been shown to be involved in the pathological process of stress-induced diarrhea. However, the role of 5-HT in stress-induced diarrhea remains unclear. A stress-induced diarrhea model was established in 21-day-old ICR weaning mice through an intragastric administration of 0.25 mL of 0.4 g/mL folium sennae and restraint of the hind legs with adhesive tape for 4 h to determine whether 5-HT regulates the mucosal barrier to cause diarrhea. Mice with decreased levels of 5-HT were pretreated with an intraperitoneal injection of 300 mg/kg p-chlorophenylalanine (PCPA), a 5-HT synthesis inhibitor. After 5 days of treatment, the stress level, body weight and intestinal mucosal morphology indexes were measured. Compared to the controls, the mice with stress-induced diarrhea displayed a stress reaction, with increased corticosterone levels, as well as increased 5-HT-positive cells. However, the mice with stress-induced diarrhea exhibited decreased body weights, villus height to crypt depth ratios (V/C), and Occludin and Claudin1 expression. The PCPA injection reversed these effects in mice with different degrees of stress-induced diarrhea. Based on these findings, inhibition of 5-HT synthesis relieved the stress response and improved the health of the intestinal tract, including both the intestinal absorption capacity, as determined by the villus height and crypt depth, and the mucosal barrier function, as determined by the tight junction proteins of epithelial cell.

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