Characterization and Mutational Analysis of Two UDP-Galactose 4-Epimerases in Streptococcus pneumoniae TIGR4

文献类型: 外文期刊

第一作者: Chen, L. L.

作者: Chen, L. L.;Han, D. L.;Zhai, Y. F.;Chen, M.;Chen, L. L.;Wang, J. H.;Wang, Y. F.

作者机构:

关键词: UDP-galactose 4-epimerase (GalE);Streptococcus pneumoniae TIGR4;mutation;substrate specificity

期刊名称:BIOCHEMISTRY-MOSCOW ( 影响因子:2.487; 五年影响因子:2.57 )

ISSN: 0006-2979

年卷期: 2018 年 83 卷 1 期

页码:

收录情况: SCI

摘要: Current clinical treatments for pneumococcal infections have many limitations and are faced with many challenges. New capsular polysaccharide structures must be explored to cope with diseases caused by different serotypes of Streptococcus pneumoniae. UDP-galactose 4-epimerase (GalE) is an essential enzyme involved in polysaccharide synthesis. It is an important virulence factor in many bacterial pathogens. In this study, we found that two genes (galE(sp1) and galE(sp2)) are responsible for galactose metabolism in pathogenic S. pneumoniae TIGR4. Both GalE(Sp1) and GalE(Sp2) were shown to catalyze the epimerization of UDP-glucose (UDP-Glc)/UDP-galactose (UDP-Gal), but only GalESp2 was shown to catalyze the epimerization of UDP-N-acetylglucosamine (UDP-GlcNAc)/UDP-N-acetylgalactosamine (UDP-GalNAc). Interestingly, GalE(Sp2) had 3-fold higher epimerase activity toward UDP-Glc/UDP-Gal than GalE(Sp1). The biochemical properties of GalE(Sp2) were studied. GalE(Sp2) was stable over a wide range of temperatures, between 30 and 70 degrees C, at pH 8.0. The K86G substitution caused GalE(Sp2) to lose its epimerase activity toward UDP-Glc and UDP-Gal; however, substitution C300Y in GalE(Sp2) resulted in only decreased activity toward UDP-GlcNAc and UDP-GalNAc. These results indicate that the Lys86 residue plays a critical role in the activity and substrate specificity of GalE(Sp2).

分类号:

  • 相关文献

[1]Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation. Tian, Jian,Wang, Ping,Gao, Shan,Chu, Xiaoyu,Wu, Ningfeng,Fan, Yunliu.

[2]Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.). Liu, Qing-Long,Xu, Xiu-Hong,Ren, Xue-Liang,Fu, Hao-Wei,Wu, Dian-Xing,Shu, Qing-Yao.

[3]Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA: diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis. Zhang, Nannan,Mao, Zejing,Luo, Ling,Wan, Xia,Gong, Yangmin,Huang, Fenghong. 2017

[4]Characterization of three Delta 9-fatty acid desaturases with distinct substrate specificity from an oleaginous fungus Cunninghamella echinulata. Wan, Xia,Liang, Zhuo,Gong, Yangmin,Zhang, Yinbo,Jiang, Mulan.

[5]The tandemly repeated domains of a beta-propeller phytase act synergistically to increase catalytic efficiency. Li, Zhongyuan,Huang, Huoqing,Yang, Peilong,Yuan, Tiezheng,Shi, Pengjun,Zhao, Junqi,Meng, Kun,Yao, Bin.

[6]Substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4 towards galactose, glucose, and their derivatives. Wang, Wenjun,Shen, Jie,Wang, Wenjun,Shen, Jie,Zou, Yang,Xue, Mengyang,Zhang, Xiaomei,Chen, Min,Zou, Yang,Xue, Mengyang,Zhang, Xiaomei,Chen, Min,Cai, Li,Chen, Leilei.

[7]Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4. Chen, Min,Chen, Lei-lei,Zou, Yang,Xue, Mengyang,Liang, Min,Jin, Lan,Guan, Wan-yi,Wang, Lei,Liu, Jun,Wang, Peng George,Chen, Min,Chen, Lei-lei,Zou, Yang,Xue, Mengyang,Liang, Min,Jin, Lan,Guan, Wan-yi,Wang, Lei,Liu, Jun,Wang, Peng George,Chen, Lei-lei,Shen, Jie,Wang, Wenjun,Wang, Peng George,Wang, Peng George.

[8]Molecular characterization of novel haplotypes of eIF4E family in Chinese cabbage (Brassica rapa L. ssp pekinensis). Liu, Shuan-Tao,Zhang, Zhi-Gang,Li, Qiao-Yun,Wang, Shu-Fen,Zhao, Zhi-Zhong,Lu, Jin-Dong,Xu, Wen-Ling,Liu, Xian-Xian,Fu, Wei-Min. 2013

[9]The mutation in nicotinic acetylcholine receptor beta 1 subunit may confer resistance to imidacloprid in Aphis gossypii (Glover). Shi, Xu-Gen,Zhu, Yu-Kun,Xia, Xiao-Ming,Qiao, Kang,Wang, Kai-Yun,Wang, Hong-Yan. 2012

[10]Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site. Qian Li-li,Cai Chun-bo,An Xiao-rong,Ma De-zun,Gao Peng-fei,Jiang Sheng-wang,Wang Qing-qing,Xiao Gao-jun,Cui Wen-tao. 2016

[11]Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed. Hu Mao-long,Pu Hui-ming,Gao Jian-qin,Long Wei-hua,Chen Feng,Zhou Xiao-ying,Zhang Wei,Peng Qi,Chen Song,Zhang Jie-fu. 2017

[12]Density alteration of nutrient elements in rice grains of a low phytate mutant. Ren, Xue-Liang,Liu, Qing-Long,Fu, Hao-Wei,Wu, Dian-xing,Shu, Qing-Yao. 2007

[13]Rifampicin-Resistance Mutations in the rpoB Gene in Bacillus velezensis CC09 have Pleiotropic Effects. Cai, Xun-Chao,Xi, Huan,Liang, Li,Liu, Jia-Dong,Liu, Chang-Hong,Xue, Ya-Rong,Yu, Xiang-Yang. 2017

[14]Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Wu, XM,Wu, NF,Qian, XZ,Li, RG,Huang, FH,Zhu, L. 1998

[15]A Rapid and Cost Effective Method for Genotyping Two Types of GHR Mutations in Sex-Linked Dwarf Chicken. Shu, Ding-Ming,Wang, Yan,Qu, Hao,Luo, Cheng-Long,Wang, Jie,Shu, Ding-Ming. 2012

[16]Warfarin resistance in Rattus losea in Guangdong Province, China. Wang, Hanshe,Zhong, Wenqin,Li, Ming,Dai, Jiayin,Feng, Zhiyong,Yao, Dandan,Sui, Jingjing. 2008

[17]Phenotypic characterization and genetic analysis of a partially female-sterile mutant in Brassica napus. Li, Chun-Hong,Fu, San-Xiong,Chen, Xin-Jun,Qi, Cun-Kou. 2012

[18]The impact of positive selection and mutation in prevalence of representative O/Mya-98 foot and mouth disease strains during 2009-2010. Kuang, Wendong,Bai, Xingwen,Qi, Guocai,Hao, Xiaofang,Li, Pinghua,Lu, Zengjun,Bao, Huifang,Sun, Pu,Wu, Lei,Liu, Zaixin. 2012

[19]The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Wang, Guozeng,Wu, Jingjing,Lin, Juan,Ye, Xiuyun,Yao, Bin.

[20]Molecular cloning and function analysis of the stay green gene in rice. Jiang, Huawu,Li, Meiru,Liang, Naiting,Yan, Hongbo,Wei, Yubo,Xu, Xinlan,Liu, Jian,Xu, Zhifang,Chen, Fan,Wu, Guojiang.

作者其他论文 更多>>