The integrity of PRRSV nucleocapsid protein is necessary for up-regulation of optimal interleukin-10 through NF-kappa B and p38 MAPK pathways in porcine alveolar macrophages

文献类型: 外文期刊

第一作者: Liu, Yanyan

作者: Liu, Yanyan;Zhang, Yuyu;Zhu, Xiwang;Ren, Sufang;Guo, Lihui;Sun, Wenbo;Chen, Zhi;Cong, Xiaoyan;Chen, Lei;Du, Yijun;Li, Jun;Wu, Jiaqiang;Wang, Jinbao;Yu, Jiang;Liu, Yanyan;Zhang, Yuyu;Zhu, Xiwang;Ren, Sufang;Guo, Lihui;Sun, Wenbo;Chen, Zhi;Cong, Xiaoyan;Chen, Lei;Shi, Jianli;Du, Yijun;Li, Jun;Wu, Jiaqiang;Wang, Jinbao;Liu, Yanyan;Zhu, Xiwang;Wu, Jiaqiang;Wang, Jinbao;Liu, Xing

作者机构:

关键词: PRRSV;Nucleocapsid;Interleukin-10;Immunosuppression;Porcine alveolar macrophages

期刊名称:MICROBIAL PATHOGENESIS ( 影响因子:3.738; 五年影响因子:3.663 )

ISSN: 0882-4010

年卷期: 2017 年 109 卷

页码:

收录情况: SCI

摘要: Porcine reproductive and respiratory syndrome (PRRS), a highly contagious disease, has been constantly causing huge economic losses all over the world. PRRS virus (PRRSV) infection results in immunosuppression and IL-10 up-regulation. The relationship between them is still in dispute. Previous studies demonstrated the protein of PRRSV nucleocapsid (N) protein is able to up-regulate IL-10, yet the underlying molecular mechanisms remain unknown. In this study, the expression kinetics of IL-10 up regulation induced by PRRSV N protein were analyzed in immortalized porcine alveolar macrophages (PAM5). N protein induced IL-10 expression in a time- and dose-dependent manner. Inhibition experiments of signaling pathways suggested NF-kappa B and p38 MAPK pathways are both involved in N protein induced IL-10 up-regulation. Besides, the integrity of N protein is essential for significant IL-10 up regulation. This research is beneficial for further understanding of the interplay between PRRSV and host immune system.(C) 2017 Published by Elsevier Ltd.

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