The K+/H+ Antiporter AhNHX1 Improved Tobacco Tolerance to NaCl Stress by Enhancing K+ Retention

文献类型： 外文期刊

第一作者： Zhang, Wei-Wei

作者： Zhang, Wei-Wei;Meng, Jing-Jing;Yang, Sha;Guo, Feng;Li, Xin-Guo;Xing, Jin-Yi;Wan, Shu-Bo

作者机构：

关键词： Arachis hypogaea L.;K+/H+ Antiporter;K+ transport;K+ uptake;Salt stress

期刊名称：JOURNAL OF PLANT BIOLOGY （ 影响因子：2.434； 五年影响因子：2.455 ）

ISSN： 1226-9239

年卷期： 2017 年 60 卷 3 期

页码：

收录情况： SCI

摘要： High salinity is the one of important factors limiting plant growth and crop production. Many NHX-type antiporters have been reported to catalyze K+/H+ exchange to mediate salt stress. This study shows that an NHX gene from Arachis hypogaea L. has an important role in K+ uptake and transport, which affects K+ accumulation and plant salt tolerance. When overexpressing AhNHX1, the growth of tobacco seedlings is improved with longer roots and a higher fresh weight than the wild type (WT) under NaCl treatment. Meanwhile, when exposed to NaCl stress, the transgenic seedlings had higher K+/H+ antiporter activity and their roots got more K+ uptake. NaCl stress could induce higher K+ accumulation in the roots, stems, and leaves of transgenic tobacco seedlings but not Na+ accumulation, thus, leading to a higher K+/Na+ ratio in the transgenic seedlings. Additionally, the AKT1, HAK1, SKOR, and KEA genes, which are involved in K+ uptake or transport, were induced by NaCl stress and kept higher expression levels in transgenic seedlings than in WT seedlings. The H+-ATPase and H+-PPase activities were also higher in transgenic seedlings than in the WT seedlings under NaCl stress. Simultaneously, overexpression of AhNHX1 increased the relative distribution of K+ in the aerial parts of the seedlings under NaCl stress. These results showed that AhNHX1 catalyzed the K+/H+ antiporter and enhanced tobacco tolerance to salt stress by increasing K+ uptake and transport.

分类号：

  • 相关文献

[1]Overexpression of an Aeluropus littoralis Parl. potassium transporter gene, AlHAK1, in cotton enhances potassium uptake and salt tolerance. Liu, J. F.,Zhang, S. L.,Tang, H. L.,Dong, L. J.,Wu, L. Z.,Liu, L. D.,Che, W. L..

[2]EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.). Li, Meng Jun,Xiao, Han,Wang, Xing Jun,Xia, Han,Zhao, Chuan Zhi,Bi, Yu Ping,Tang, Rong Hua. 2010

[3]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[4]Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.. Li, Meng-Jun,Li, Ai-Qin,Xia, Han,Zhao, Chuan-Zhi,Li, Chang-Sheng,Wan, Shu-Bo,Bi, Yu-Ping,Wang, Xing-Jun. 2009

[5]Cloning and expression analysis of peanut (Arachis hypogaea L.) CHI gene. Zhang, Yue,Wang, Xingjun,Zhang, Yue,Xia, Han,Zhao, Chuanzhi,Li, Aiqin,Wang, Xingjun,Yuan, Mei. 2012

[6]Isolation and expression analysis of LEA genes in peanut (Arachis hypogaea L.). Su, Lei,Bi, Yu-Pin,Wang, Xing-Jun,Su, Lei,Zhao, Chuan-Zhi,Bi, Yu-Pin,Wan, Shu-Bo,Xia, Han,Wang, Xing-Jun,Su, Lei,Zhao, Chuan-Zhi,Bi, Yu-Pin,Wan, Shu-Bo,Xia, Han,Wang, Xing-Jun,Su, Lei,Zhao, Chuan-Zhi,Bi, Yu-Pin,Wan, Shu-Bo,Xia, Han,Wang, Xing-Jun.

[7]Agrobacterium-mediated genetic transformation of peanut and the efficient recovery of transgenic plants. Chen, Mingna,Yang, Qingli,Wang, Tong,Chen, Na,Pan, Lijuan,Chi, Xiaoyuan,Yang, Zhen,Wang, Mian,Yu, Shanlin,Chi, Xiaoyuan. 2015

[8]Controlled Release Study on Microencapsulated Mixture of Fipronil and Chlorpyrifos for the Management of White Grubs (Holotrichia parallela) in Peanuts (Arachis hypogaea L.). Yang, Daibin,Yan, Xiaojing,Yuan, Huizhu,Li, Guangxing. 2014

[9]Expressed sequence tags in cultivated peanut (Arachis hypogaea): Discovery of genes in seed development and response to Ralstonia solanacearum challenge. Huang, Jiaquan,Yan, Liying,Lei, Yong,Jiang, Huifang,Ren, Xiaoping,Liao, Boshou.

[10]Diversity characterization and association analysis of agronomic traits in a Chinese peanut (Arachis hypogaea L.) mini-core collection. Jiang, Huifang,Huang, Li,Ren, Xiaoping,Chen, Yuning,Zhou, Xiaojing,Huang, Jiaquan,Lei, Yong,Yan, Liying,Wan, Liyun,Liao, Boshou,Xia, Youlin. 2014

[11]Assessment of Photochemical Reflectance Index as a Tool for Evaluation of Chlorophyll Fluorescence Parameters in Cotton and Peanut Cultivars Under Water Stress Condition. Shahenshah,Akihiro, Isoda,Yoshizumi, Yasuda,Li Mao-song. 2010

[12]Phytotoxicity of Silver Nanoparticles to Peanut (Arachis hypogaea L.): Physiological Responses and Food Safety. Rui, Mengmeng,Yang, Jie,Jiang, Fuping,Pan, Yue,Xiang, Zhiqian,Hao, Yi,Rui, Yukui,Rui, Mengmeng,Tang, Xinlian,Ma, Chuanxin,Xing, Baoshan,Ma, Chuanxin,Cao, Weidong,Ma, Chuanxin. 2017

[13]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[14]Cloning of the full-length cDNA of the wheat involved in salt stress: Root hair defective 3 gene (RHD3). Shan, L,Zhao, SY,Xia, GM. 2005

[15]Molecular cloning and expression analysis of FvMYB1 from Fraxinus velutina Torr.. Li, Tian,Bi, Yu-Ping,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian,Peng, Zhen-Ying,Bi, Yu-Ping,Fan, Zhong-Xue,Li, Tian. 2013

[16]Genome-wide identification of Thellungiella salsuginea microRNAs with putative roles in the salt stress response. Zhang, Quan,Sun, Wei,Liu, Yan,Wang, Xingjun,Zhao, Yanxiu,Zhao, Chuanzhi,Li, Ming,Xia, Han,Sun, Mingnan,Li, Aiqin,Li, Changsheng,Zhao, Shuzhen,Hou, Lei,Picimbon, Jean-Francois,Wang, Xingjun. 2013

[17]A R2R3 MYB transcription factor from ash positively regulates salt response in tobacco. Li, Tian,Sun, Jingkuan,Li, Tian,Bi, Yuping. 2017

[18]Identification of Metabolites and Transcripts Involved in Salt Stress and Recovery in Peanut. Cui, Feng,Liu, Yiyang,Han, Yan,Wan, Shubo,Li, Guowei,Cui, Feng,Liu, Yiyang,Han, Yan,Wan, Shubo,Li, Guowei,Sui, Na,Liu, Shanshan,Duan, Guangyou. 2018

[19]Global Analysis of WRKY Genes and Their Response to Dehydration and Salt Stress in Soybean. Song, Hui,Wang, Pengfei,Hou, Lei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Li, Pengcheng,Zhang, Ye,Bian, Xiaotong,Wang, Xingjun. 2016

[20]Exogenous nitric oxide delays salt-induced leaf senescence in cotton (Gossypium hirsutum L.). Kong, Xiangqiang,Wang, Tao,Li, Weijiang,Tang, Wei,Zhang, Dongmei,Dong, Hezhong,Wang, Tao,Dong, Hezhong.

作者其他论文 更多>>

微信小程序