-catenin promotes intracellular bacterial killing via suppression of Pseudomonas aeruginosa-triggered macrophage autophagy

文献类型： 外文期刊

第一作者： Fu, Qiang

作者： Fu, Qiang;Chen, Kang;Wang, Weijia;Huang, Fuda;Miao, Lishao;Wu, Xinger;Zhu, Qian

作者机构：

关键词： Pseudomonas aeruginosa;-catenin;bacterial killing;autophagy

期刊名称：JOURNAL OF INTERNATIONAL MEDICAL RESEARCH （ 影响因子：1.671； 五年影响因子：1.805 ）

ISSN： 0300-0605

年卷期： 2017 年 45 卷 2 期

页码：

收录情况： SCI

摘要： Objective To investigate -catenin-mediated bacterial elimination during Pseudomonas aeruginosa infection of macrophage-like RAW264.7 cells. Methods Cell viability and catenin beta 1 (CTNNB1) expression in RAW264.7 cells following P. aeruginosa infection versus uninfected cells, were detected by cell counting kit-8 assay and -catenin Western blots. RAW264.7 cells with CTNNB1 overexpression were established with -catenin lentivirus using flow cytometry and clonogenic limiting dilution assays. Bacterial killing was measured by plate counts; phagocytosis and nitric oxide (NO) were measured by flow cytometry; and reactive oxygen species (ROS) were measured using Griess reaction. Autophagy was determined by microtubule-associated protein 1 light chain 3 alpha-phosphatidylethanolamine conjugate (LC3-II) protein levels and formation of LC3 puncta, using Western blot and immunofluorescence staining. Results Following P. aeruginosa infection, RAW264.7 cell -catenin levels were reduced in a time- and multiplicity of infection-dependent manner. CTNNB1 overexpression was associated with increased P. aeruginosa elimination, but had no effect on RAW264.7 cell phagocytosis, ROS and NO. CTNNB1 overexpression reduced LC3-II levels and formation of LC3 puncta, suggesting autophagy inhibition. Rapamycin/starvation-induced autophagy resulted in reduced bacterial killing following P. aeruginosa infection. Conclusion -catenin may promote bacterial killing via suppression of P. aeruginosa-induced macrophage autophagy.

分类号：

  • 相关文献

[1]Anti-phytopathogens Activity of Antibiotic Compound F125 (Phenazine-1-carboxamide) from a Pseudomonas aeruginosa Strain SU8. Liu, S. Q.,Wang, C.,Su, P.,Liao, X. L.. 2016

[2]Expression of mucoid induction factor MucE is dependent upon the alternate sigma factor AlgU in Pseudomonas aeruginosa. Yin, Yeshi,Damron, F. Heath,Withers, T. Ryan,Yu, Hongwei D.,Yu, Hongwei D.,Yin, Yeshi,Wang, Xin,Yu, Hongwei D.,Pritchett, Christopher L.,Schurr, Michael J.,Pritchett, Christopher L.,Damron, F. Heath. 2013

[3]Characterization of cadmium-resistant bacteria and their potential for reducing accumulation of cadmium in rice grains. Chen, Mingxue.

[4]A method for efficient expression of Pseudomonas aeruginosa alginate lyase in Pichia pastoris. Qiao, Yu,Ding, Hongbiao,Gong, Wendy W.. 2016

[5]Rapid detection of Pseudomonas aeruginosa septicemia by real-time fluorescence loop-mediated isothermal amplification of free DNA in blood samples. Yang, Rungong,Fu, Shuhong,Gong, Meiliang,Li, Xiaoxia,Yu, Jihong,Bai, Jie,Ye, Ling,Yang, Jinghui,Chen, Hongyan,Fan, Yanfeng. 2016

[6]Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production. Yan, Yanchun,Zhang, Chen,Wang, Shenghui.

[7]Pseudomonas aeruginosa as a Powerful Biofilm Producer and Positive Action of Amikacin Against Isolates From Chronic Wounds. Rahim, Kashif,Zhu, Xudong,Huo, Liang,Zhang, Ping,Saleha, Shamim,Basit, Abdul,Ahmed, Iqbal,Usman, Bakhtawar,Munir, Shahzad,Franco, Octavio Luiz,Franco, Octavio Luiz. 2017

[8]Antagonistic Activity and Mode of Action of Phenazine-1-Carboxylic Acid, Produced by Marine Bacterium Pseudomonas aeruginosa PA31x, Against Vibrio anguillarum In vitro and in a Zebrafish In vivo Model. Zhang, Linlin,Kuang, Shan,Liu, Ge,Sun, Chaomin,Zhang, Linlin,Kuang, Shan,Liu, Ge,Sun, Chaomin,Zhang, Linlin,Liu, Ge,Tian, Xueying,Zhang, Chengsheng. 2017

[9]The identification, typing, and antimicrobial susceptibility of Pseudomonas aeruginosa isolated from mink with hemorrhagic pneumonia. Qi, Jing,Li, Lulu,Du, Yijun,Wang, Jinwen,Luo, Yanbo,Liu, Yuqing,Qi, Jing,Du, Yijun,Wang, Jinwen,Wang, Guisheng,Hu, Ming,Yan, Shigan,Liu, Yuqing,Wang, Shourong,Che, Jie,Lu, Jinxing,Liu, Hui,Hu, Guangchun,Li, Jixia,Gong, Yanwen,Wang, Guisheng.

[10]Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Guo, X.,Dong, Y.,Yin, S.,Zhao, C.,Huo, Y.,Hu, H.,Guo, X.,Fan, L.. 2013

[11]Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Meng, Gang,Xia, Mao,Wang, Diancheng,Chen, Aiping,Wang, Hongwei,Yu, Decai,Wei, Jiwu,Yu, Decai,Wei, Jiwu,Wang, Yongshan. 2014

[12]Rice Stripe Virus Interferes with S-acylation of Remorin and Induces Its Autophagic Degradation to Facilitate Virus Infection. Xu, Yi,Li, Chenyang,Wu, Jianxiang,Zhou, Xueping,Li, Chenyang,Zhou, Xueping,Li, Yi,Xu, Yi. 2018

[13]Autophagy Plays an Important Role in Anti-inflammatory Mechanisms Stimulated by Alpha7 Nicotinic Acetylcholine Receptor. Shao, Bo-Zong,Ke, Ping,Xu, Zhe-Qi,Cheng, Ming-He,Han, Bin-Ze,Su, Ding-Feng,Liu, Chong,Wei, Wei,Chen, Xiong-Wen. 2017

[14]Regulation of ATG6/Beclin-1 homologs by abiotic stresses and hormones in rice (Oryza sativa L.). Rana, R. M.,Dong, S.,Huang, J.,Zhang, H. S.,Rana, R. M.,Ali, Z.,Ali, Z.. 2012

[15]3 '-epi-12 beta-hydroxyfroside, a new cardenolide, induces cytoprotective autophagy via blocking the Hsp90/Akt/mTOR axis in lung cancer cells. Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Sun, Yan,Huang, Yong-Hao,Huang, Feng-Ying,Wang, Cai-Chun,Lin, Ying-Ying,Huang, Canhua,Li, Yue-Nan,Tan, Guang-Hong,Mei, Wen-Li,Dai, Hao-Fu,Liu, Quan,Liu, Quan,Huang, Canhua,Li, Yue-Nan. 2018

[16]Activating Cannabinoid Receptor 2 Alleviates Pathogenesis of Experimental Autoimmune Encephalomyelitis Via Activation of Autophagy and Inhibiting NLRP3 Inflammasome. Shao, Bo-Zong,Ke, Ping,Xu, Zhe-Qi,Zhou, Jv-Xiang,Liu, Chong,Wei, Wei. 2014

[17]Caspase-1 participates in apoptosis of salivary glands in Rhipicephalus haemaphysaloides. Yu, Xinmao,Zhou, Yongzhi,Cao, Jie,Zhang, Houshuang,Gong, Haiyan,Zhou, Jinlin,Zhou, Jinlin. 2017

[18]Autophagy regulates spermatid differentiation via degradation of PDLIM1. Shang, Yongliang,Wang, Hongna,Zhao, Haichao,Liu, Chao,Liu, Weixiao,Song, Zhenhua,Xu, Zhiliang,Li, Wei,Shang, Yongliang,Wang, Hongna,Zhao, Haichao,Liu, Chao,Song, Zhenhua,Xu, Zhiliang,Jia, Pengfei,Yang, Lin,Jia, Pengfei,Yang, Lin,Wang, Yanfang.

[19]Oleanolic acid inhibits proliferation and invasiveness of Kras-transformed cells via autophagy. Liu, Jia,Zheng, Lanhong,Liu, Jia,Wu, Ning,Liu, Ge,Lin, Xiukun,Ma, Leina,Wang, Bin,Zhao, Youguang,Lin, Xiukun.

[20]Infectious Bursal Disease Virus Subverts Autophagic Vacuoles To Promote Viral Maturation and Release. Wang, Yongqiang,Duan, Yulu,Han, Chunyan,Yao, Shuai,Qi, Xiaole,Gao, Yulong,Zhang, Lizhou,Gao, Li,Gao, Honglei,Shen, Nan,Wang, Jingfei,Wang, Xiaomei,Maier, Helena J.,Britton, Paul,Chen, Lei.

作者其他论文 更多>>

微信小程序