Construction and characterization of a bacterial artificial chromosome library of the maize inbred line Qi319

文献类型： 外文期刊

第一作者： Mu, Chun Hua

作者： Mu, Chun Hua;Zhang, Fa Jun;Li, Wen Cai;Lu, Shou Ping;Meng, Zhao Dong;Liu, Xia;Mu, Chun Hua;Liu, Xia;Yang, Yu;Li, Guang Cun

作者机构：

关键词： Maize;inbred line;resistance genes;bacterial artificial chromosome library

期刊名称：CROP BREEDING AND APPLIED BIOTECHNOLOGY （ 影响因子：1.282； 五年影响因子：1.493 ）

ISSN： 1984-7033

年卷期： 2016 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Zea mays L. has been the most cultivated crop and the crop with the largest yield in China since 2012. We constructed a bacterial artificial chromosome (BAC) library for the maize inbred line Qi319, which may be used as a key source for disease-resistant maize breeding in China. The BAC contains 270,720 clones, with an average insert size of 90 kb. The coverage of the library is about 10.43 genome equivalents when considering a haploid genome size of 2300 Mb, providing a 99.99% likelihood of isolating any maize gene or sequence in the library. An average of 12 clones were obtained by polymerase chain reaction screening by using primer pairs linked to the genes for resistance to maize southern rust and rough dwarf. The results indicate that the library can satisfy the requirements for recovering specific sequences. The library is available to researchers to whom it may be of interest.

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