Comparative Transcriptomes Analysis of Red- and White-Fleshed Apples in an F-1 Population of Malus sieversii f. niedzwetzkyana Crossed with M. domestica 'Fuji'

文献类型： 外文期刊

第一作者： Wang, Nan

作者： Wang, Nan;Duan, Naibin;Zhang, Zongying;Ji, Xiaohao;Jiang, Shenghui;Sun, Shasha;Yang, Long;Chen, Xuesen;Wang, Nan;Duan, Naibin;Zhang, Zongying;Ji, Xiaohao;Jiang, Shenghui;Sun, Shasha;Yang, Long;Chen, Xuesen;Zheng, Yi;Bai, Yang;Fei, Zhangjun;Duan, Naibin;Yang, Long

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 7 期

页码：

收录情况： SCI

摘要： Transcriptome profiles of the red- and white-fleshed apples in an F-1 segregating population of Malus sieversii f. Niedzwetzkyana and M. domestica 'Fuji' were generated using the next-generation high-throughput RNA sequencing (RNA-Seq) technology and compared. A total of 114 differentially expressed genes (DEGs) were obtained, of which 88 were up-regulated and 26 were down-regulated in red-fleshed apples. The 88 up-regulated genes were enriched with those related to flavonoid biosynthetic process and stress responses. Further analysis identified 22 genes associated with flavonoid biosynthetic process and 68 genes that may be related to stress responses. Furthermore, the expression of 20 up-regulated candidate genes (10 related to flavonoid biosynthesis, two encoding MYB transcription factors and eight related to stress responses) and 10 down-regulated genes were validated by quantitative real-time PCR. After exploring the possible regulatory network, we speculated that flavonoid metabolism might be involved in stress responses in red-fleshed apple. Our findings provide a theoretical basis for further enriching gene resources associated with flavonoid synthesis and stress responses of fruit trees and for breeding elite apples with high flavonoid content and/or increased stress tolerances.

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