Inducing autotetraploid and developing a rapid propagation strategy for Codonopsis pilosula

文献类型： 外文期刊

第一作者： Gao, Jin-Rong

作者： Gao, Jin-Rong;Ren, Yan;Huang, Heng-Yu;Yan, Hong-Mei

作者机构：

关键词： Codonopsis pilosula; Autotetraploid; In vitro rapid propagation; Rooting; Morphological analysis

期刊名称：CYTOLOGIA （ 影响因子：0.4； 五年影响因子：0.5 ）

ISSN： 0011-4545

年卷期： 2024 年 89 卷 3 期

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收录情况： SCI

摘要： Codonopsis pilosula (Franch.) Nannf. is used as root organ medicine, and ploidy breeding is conducive to its resource development and utilization. The autotetraploid of C. pilosula was induced by colchicine with seeds and sterile stem tips as materials and identified by flow cytometry and chromosome counting. At the same time, based on the diploid proliferation medium, the L-9 (3(4)) orthogonal experiment was carried out with 6-benzylaminopurine (6-BA), alpha-naphthaleneacetic acid (NAA), and kinetin (KT) as factors to optimize the autotetraploid proliferation medium and realize rooting outside the bottle. Finally, the morphological cell analysis of the autotetraploid was carried out. The results showed that stem tips were more suitable for inducing tetraploid than the seeds. The chromosome number of diploid was 2n=2x=16, and that of tetraploid was 2n=4x=32. Adventitious buds were identified by three consecutive generations, and no ploidy variation appeared. The callus induction and adventitious bud proliferation of the tetraploid stem tip were synchronized in Murashige and Skoog (MS) medium with 60 mL L-1 coconut juice, 1.0 mg L-1 6-BA, 0.5 mg L-1 NAA, and 0.1 mg L-1 KT, and the proliferation coefficient was up to 39.69. The rooting rate of tetraploid-regenerated buds outside the bottle was 85.97%, and the survival rate after transplanting was 98.26%. Tetraploid plants exhibited superior characteristics in terms of leaf size, stem diameter, number of branches and leaves, and floral organ size when compared to diploid plants. In addition, the induction method of diploid stem tips soaked in a colchicine sterile solution and then cultured in the colchicine-containing medium greatly improved the induction rate of tetraploid. In this study, the autotetraploid of C. pilosula was successfully induced, and the artificial efficient regeneration system of tetraploid C. pilosula was established, which provided an effective way for the optimization of C. pilosula germplasm resources.

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