Genetically engineering Synechocystis sp Pasteur Culture Collection 6803 for the sustainable production of the plant secondary metabolite p-coumaric acid

文献类型： 外文期刊

第一作者： Xue, Yong

作者： Xue, Yong;Cheng, Dan;Daddy, Soumana;He, Qingfang;Zhang, Yan;He, Qingfang

作者机构：

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.205； 五年影响因子：12.291 ）

ISSN： 0027-8424

年卷期： 2014 年 111 卷 26 期

页码：

收录情况： SCI

摘要： p-Coumaric acid is the precursor of phenylpropanoids, which are plant secondary metabolites that are beneficial to human health. Tyrosine ammonia lyase catalyzes the production of p-coumaric acid from tyrosine. Because of their photosynthetic ability and biosynthetic versatility, cyanobacteria are promising candidates for the production of certain plant metabolites, including phenylpropanoids. Here, we produced p-coumaric acid in a strain of transgenic cyanobacterium Synechocystis sp. Pasteur Culture Collection 6803 (hereafter Synechocystis 6803). Whereas a strain of Synechocystis 6803 genetically engineered to express sam8, a tyrosine ammonia lyase gene from the actinomycete Saccharothrix espanaensis, accumulated little or no p-coumaric acid, a strain that both expressed sam8 and lacked slr1573, a native hypothetical gene shown here to encode a laccase that oxidizes polyphenols, produced similar to 82.6 mg/L p-coumaric acid, which was readily purified from the growth medium.

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