In vitro and in vivo replication of influenza A RI NI WSN33 viruses with different M1 proteins

文献类型： 外文期刊

第一作者： Ran, Zhiguang

作者： Ran, Zhiguang;Xiang, Xiaoxiao;Zhu, Laihua;Li, Feng;Ran, Zhiguang;Xiang, Xiaoxiao;Zhu, Laihua;Young, Alan;Li, Feng;Ran, Zhiguang;Chen, Ying;Shen, Huigang;Liu, Qinfang;Bawa, Bhupinder;Qi, Wenbao;Richt, Juergen;Ma, Wenjun;Li, Feng

作者机构：

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN： 0022-1317

年卷期： 2013 年 94 卷

页码：

收录情况： SCI

摘要： The M1 protein is a major structural protein that has multiple functions in various steps within the life cycle of the influenza A virus (IAV). However, little is currently known about the role of M1 in IAV replication in vivo and the associated pathogenesis. In this study, six isogenic H1N1 WSN33 viruses, constructed to express unique M1 proteins derived from various strains, subtypes or WSN33 itself, were tested to determine in vitro and in vivo functional exchangeability of M1 proteins in the replication and pathogenesis of the WSN33 virus. Despite five chimeric M1 viruses replicating to levels similar to those of the parental WSN33 virus in cell cultures, all M1 chimeras exhibited improved replication and enhanced virulence in mice when compared with the WSN33 virus. Interestingly, M1 proteins derived from swine viruses caused more severe clinical diseases than those from human or quail. These data indicate that the M1 protein is an important determinant of viral replication and pathogenic properties in mice, although the functions of M1 observed in vivo are not adequately reflected in simple infections of cultured cells. Chimeric M1 viruses that are variable in their clinical manifestations described here will aid future understanding of the role of M1 in IAV pathogenesis.

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