Differential gene expression of bovine intramuscular and subcutaneous preadipocytes during differentiation

文献类型: 外文期刊

第一作者: Liu, Xiaomu

作者: Liu, Xiaomu;Song, Enliang;Tan, Xiuwen;Liu, Guifen;Cheng, Haijian;Wan, Fachun;Liu, Xiaomu;Song, Enliang;Tan, Xiuwen;Liu, Guifen;Cheng, Haijian;Wan, Fachun;Zang, Kun;Ling, Zongshuai

作者机构:

关键词: Bovine preadipocyte differentiation;FoxO1;GAPDH;IGF-1;leptin;PPAR gamma;SIRT1

期刊名称:ANIMAL SCIENCE PAPERS AND REPORTS ( 影响因子:1.078; 五年影响因子:1.097 )

ISSN: 0860-4037

年卷期: 2012 年 30 卷 2 期

页码:

收录情况: SCI

摘要: The study suggests that Sirtuin 1 (SIRT1) may play an important role in the development of bovine adipose tissue in vivo. To investigate the differential mRNA expression between bovine intramuscular (IM) preadipocytes (BIPs) and subcutaneous (SC) preadipocytes (BSPs), conventional collagenase-based procedures were modified to harvest preadipocytes from the longissimus dorsi muscle and SC adipose tissue of Luxi cattle, respectively. The time-spatial expression of SIRT1 and associated genes, such as forkhead box O1(FoxO1), peroxisome proliferator-activated receptor-gamma (PPAR gamma), insulin-like growth factor-1(IGF-1), glyceraldehydes-3-phosphate dehydrogenase(GAPDH), and leptin, during bovine preadipocyte differentiation were investigated using real-time quantitative PCR. The intracellular triglyceride concentration of BSPs increased more dramatically than that of BIPs during cell differentiation. The expression of SIRT1 and other genes occurred in the BSPs and BIPs during differentiation indicating that different mechanisms underlie the adipogenesis of BSPs and BIPs, and that the BSPs cultured in vitro show a higher differentiation capacity than BIPs.

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