Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity

文献类型: 外文期刊

第一作者: Zhang, Bo

作者: Zhang, Bo;Li, Changsong;Liu, Huaiwei;Bao, Xiaoming

作者机构:

关键词: Chitosanase;Fusarium solani;RNA silencing;Pathogenicity

期刊名称:CURRENT GENETICS ( 影响因子:3.886; 五年影响因子:3.697 )

ISSN: 0172-8083

年卷期: 2010 年 56 卷 3 期

页码:

收录情况: SCI

摘要: Chitosanases are lytic enzymes involved in the degradation of chitosan, a component of fungal cell walls. The phytopathogenic fungus Fusarium solani produces an extracellular chitosanase, CSN1, the role of which in the physiology and virulence of the fungus remains to be expounded. Here, we studied the expression of the CSN1 gene through gene silencing and examined its effect on fungal pathogenicity. A vector construct encoding a hairpin RNA (hpRNA) of CSN1 was constructed and introduced into the F. solani 0114 strain. The results revealed that majority of the transformants exhibited a significant reduction in chitosanase activity compared with the wild-type strain. Further, transformants with silenced CSN1 exhibited no change in mycelial growth and spore formation. However, pea pod and seedling bioassays indicated that transformants with silenced CSN1 were more virulent compared with the wild-type strain, and in sharp contrast to strains in which overexpression of the CSN1 gene resulted in virulence reduction. Although the mechanism remains unclear, our findings did suggest that F. solani chitosanase has a negative effect on fungal pathogenicity.

分类号:

  • 相关文献

[1]Structural insights into the substrate-binding mechanism for a novel chitosanase. Lyu, Qiangian,Han, Baoqin,Liu, Wanshun,Liu, Weizhi,Wang, Song,Xu, Wenhua,Junes, David N. M..

[2]A highly Conserved Aspartic Acid Residue of the Chitosanase from Bacillus Sp TS Is Involved in the Substrate Binding. Zhou, Zhanping,Liu, Yang,Chang, Zhengying,Ma, Yanhe,Song, Jiangning,Zhou, Zhanping,Liu, Yang,Chang, Zhengying,Ma, Yanhe,Song, Jiangning,Zhao, Shuangzhi,Li, Jian,Song, Jiangning,Song, Jiangning.

[3]A high throughput method for rapid screening of chitosanase-producing fungal strain under acidic conditions. Chen, Gui-Guang,Liang, Zhi-Qun,Zeng, Wei,Chen, Gui-Guang,Liang, Zhi-Qun,Zeng, Wei,Chen, Gui-Guang,Liang, Zhi-Qun,Zeng, Wei,Cao, Mu-Ming,Chen, Guo-Pin,Xie, Shu-Yu,Li, Wei.

[4]Generation of double-virus-resistant marker-free transgenic potato plants. Bai, Yunfeng,Guo, Zhihua,Wang, Xiaoqi,Bai, Dongmei,Zhang, Weifeng. 2009

[5]Improved Pathogenicity of a Beet Black Scorch Virus Variant by Low Temperature and Co-infection with its Satellite RNA. Xu, Jin,Liu, Deshui,Zhang, Yongliang,Wang, Ying,Han, Chenggui,Li, Dawei,Yu, Jia-Lin,Wang, Xian-Bing,Xu, Jin. 2016

[6]Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum. Mao, Zhen-Chuan,Kang, Hou-Xiang,Chen, Guo-Hua,Yang, Yu-Hong,Xie, Bing-Yan,Bai, Miao,Yang, Guo-Shun,Chen, Wen-Ting.

[7]Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Inhibits RNA-Mediated Gene Silencing by Targeting Ago-2. Chen, Jing,Zhang, Gaiping,Chen, Jing,Shi, Xibao,Wang, Li,Luo, Jun,Xing, Guangxu,Deng, Ruiguang,Zhang, Gaiping,Shi, Xibao,Zhang, Xiaozhuan,Yang, Hong,Li, Jinting,Wang, Aiping,Zhang, Gaiping. 2015

[8]High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants. Shen Wan-xia,Zhou Chang-yong,Shen Wan-xia,Smith, Neil A.,Wang Ming-bo,Shen Wan-xia,Zhou Chang-yong. 2014

[9]SGS3 Cooperates with RDR6 in Triggering Geminivirus-Induced Gene Silencing and in Suppressing Geminivirus Infection in Nicotiana Benthamiana. Zhou, Xueping,Li, Fangfang,Wang, Yaqin,Zhou, Xueping. 2017

[10]Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene. Chen, Ming,Ma, Youzhi,Du, Lipu,Ye, Xingguo,Pang, Junlan,Zhang, Xinmei,Li, Liancheng,Xu, Huijun,Sun, Liying,Chen, Jiong,Andika, Ida B.,Chen, Jianping.

[11]A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks. Bai Miao,Chen Wen-ting,Yang Gun-shun,Xie Bing-yan. 2016

[12]CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis. Fang, Yuan-Yuan,Zhao, Jian-Hua,Liu, Shang-Wu,Wang, Sheng,Duan, Cheng-Guo,Guo, Hui-Shan,Liu, Shang-Wu,Guo, Hui-Shan,Duan, Cheng-Guo. 2016

[13]Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana. Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Peng, Jiejun,Chen, Hairu,Yang, Jian,Jiang, Shanshan. 2011

[14]Characterization of small interfering RNAs derived from Rice black streaked dwarf virus in infected maize plants by deep sequencing. Li, Mingjun,Li, Yongqiang,Xia, Zihao,Zhou, Tao,Fan, Zaifeng,Li, Mingjun,Li, Yongqiang,Xia, Zihao,Zhou, Tao,Fan, Zaifeng,Di, Dianping,Zhang, Aihong,Miao, Hongqin.

[15]RNA-Dependent RNA Polymerase 1 from Nicotiana tabacum Suppresses RNA Silencing and Enhances Viral Infection in Nicotiana benthamiana. Ying, Xiao-Bao,Dong, Li,Zhu, Hui,Duan, Cheng-Guo,Du, Quan-Sheng,Lv, Dian-Qiu,Fang, Yuan-Yuan,Fang, Rong-Xiang,Guo, Hui-Shan,Ying, Xiao-Bao,Dong, Li,Zhu, Hui,Duan, Cheng-Guo,Du, Quan-Sheng,Lv, Dian-Qiu,Fang, Yuan-Yuan,Fang, Rong-Xiang,Guo, Hui-Shan,Ying, Xiao-Bao,Zhu, Hui,Lv, Dian-Qiu,Antonio Garcia, Juan.

[16]RNA-dependent RNA polymerase 6 of rice (Oryza sativa) plays role in host defense against negative-strand RNA virus, Rice stripe virus. Jiang, Lin,Qian, Dan,Zheng, Hong,Meng, Lin-Yan,Wei, Chun-Hong,Li, Yi,Chen, Jie,Le, Wen-Jing,Zhou, Tong,Zhou, Yi-Jun.

[17]Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria. Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Yang, G..

[18]PB2-E627K and PA-T97I substitutions enhance polymerase activity and confer a virulent phenotype to an H6N1 avian influenza virus in mice. Cheng, Kaihui,Yu, Zhijun,Sun, Weiyang,Xin, Yue,Huang, Jing,Zhang, Kun,Li, Xue,Yang, Songtao,Wang, Tiecheng,Zheng, Xuexing,Wang, Hualei,Qian, Jun,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Yu, Zhijun,Qin, Chuan,Xia, Xianzhu,Zhang, Qianyi,Chen, Hualan,Chai, Hongliang,Hua, Yuping.

[19]Multiple amino acid substitutions involved in the virulence enhancement of an H3N2 avian influenza A virus isolated from wild waterfowl in mice. Yu, Zhijun,Sun, Weiyang,Zhang, Xinghai,Gao, Yuwei,Xia, Xianzhu,Gao, Yuwei,Xia, Xianzhu,Cheng, Kaihui,Zhao, Chuqi.

[20]Co-Infection with Marek's Disease Virus and Reticuloendotheliosis Virus Increases Illness Severity and Reduces Marek's Disease Vaccine Efficacy. Sun, Guo-Rong,Zhang, Yan-Ping,Zhou, Lin-Yi,Lv, Hong-Chao,Zhang, Feng,Li, Kai,Gao, Yu-Long,Qi, Xiao-Le,Cui, Hong-Yu,Wang, Yong-Qiang,Gao, Li,Pan, Qing,Wang, Xiao-Mei,Liu, Chang-Jun. 2017

作者其他论文 更多>>