Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV

文献类型: 外文期刊

第一作者: Chen, Changmu

作者: Chen, Changmu;Cui, Shangjin;Zhang, Chaofan;Li, Jun;Wang, Jinbao

作者机构:

关键词: Porcine reproductive and respiratory syndrome virus;Reverse-transcription loop-mediated isothermal amplification

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

ISSN: 0920-8569

年卷期: 2010 年 40 卷 1 期

页码:

收录情况: SCI

摘要: To establish a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid detection of porcine reproductive and respiratory syndrome virus (PRRSV), four primers specific to six regions of the N gene were designed. After amplification in an isothermal water bath for 1 h, samples containing PRRSV generated the expected ladder-like products while porcine parvovirus, porcine circovirus, classic swine fever virus, pseudorabies virus, and swine testis cells generated no product. The sensitivity and specificity of the RT-LAMP assay were evaluated by comparison with reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Because it is specific and simple, the RT-LAMP assay will be useful for the diagnosis of PRRSV infection.

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