High Efficiency Shoot Regeneration from Leaf Explants of Taishan Sour Jujube (Zizyphus spinosus Hu.)

文献类型： 外文期刊

第一作者： Sun, H.

作者： Sun, H.;Sun, Q.;Zhou, G.;Liu, Q.;Xin, L.

作者机构：

关键词： jujube rootstock;in vitro leaves;callus induction;bud induction;shoot elongation

期刊名称：I INTERNATIONAL JUJUBE SYMPOSIUM

ISSN： 0567-7572

年卷期： 2009 年 840 卷

页码：

收录情况： SCI

摘要： Being high resistant to cold and drought, tolerant to barren and saline-alkali, more developed roots, sour jujube is a good rootstock of cultivated jujube. Taishan sour jujube is deciduous shrub, grown on mountain Taishan. A highly efficient protocol was developed for shoot regeneration from leaf explants in Taishan sour jujube (Zizyphus spinosus Hu.). This protocol used thidiazuron (TDZ) and indole-3-acetic acid (IAA) for callus induction from leaf explants in dark culture, and used IAA and gibberellic acid (GA(3)) for shoot buds regeneration and elongation. The optimal media for shoot buds formation and elongation were the medium WPM containing 0.5-1.0 mg/L TDZ and 0.1 or 0.5 mg/L IAA in combination with the medium WPM containing 0.1 mg/L IAA and 0.5 mg/L GA(3), the highest frequency of shoot regeneration was 100%. The induction of shoot buds from leaf explants in light culture was also examined. The bud formation was observed, but in this case, the buds were smaller and directly formed from leaf explants. The dark culture of leaf explants at inducing callus step was necessary for shoot-buds to develop normally and elongate later. This regeneration protocol may be applicable to the improvement of this crop by genetic engineering in the future.

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