Systematic transcriptome-wide analysis of mRNA-miRNA interactions reveals the involvement of miR-142-5p and its target (FOXO3) in skeletal muscle growth in chickens

文献类型： 外文期刊

第一作者： Li, Zhenhui

作者： Li, Zhenhui;Abdalla, Bahareldin Ali;Zheng, Ming;He, Xiaomei;Cai, Bolin;Han, Peigong;Ouyang, Hongjia;Chen, Biao;Nie, Qinghua;Zhang, Xiquan;Li, Zhenhui;Abdalla, Bahareldin Ali;Zheng, Ming;He, Xiaomei;Cai, Bolin;Han, Peigong;Ouyang, Hongjia;Chen, Biao;Nie, Qinghua;Zhang, Xiquan;Li, Zhenhui;Abdalla, Bahareldin Ali;Zheng, Ming;He, Xiaomei;Cai, Bolin;Han, Peigong;Ouyang, Hongjia;Chen, Biao;Nie, Qinghua;Zhang, Xiquan

作者机构：

关键词： miRNA-mRNA network;miR-142-5p;FOXO3;Skeletal muscle growth

期刊名称：MOLECULAR GENETICS AND GENOMICS （ 影响因子：3.291； 五年影响因子：3.257 ）

ISSN： 1617-4615

年卷期： 2018 年 293 卷 1 期

页码：

收录情况： SCI

摘要： The goal of this study was to perform a systematic transcriptome-wide analysis of mRNA-miRNA interactions and to identify candidates involved in the interplay between miRNAs and mRNAs that regulate chicken muscle growth. We used our previously published mRNA (GSE72424) and miRNA (GSE62971) deep sequencing data from two-tailed samples [i.e., the highest (h) and lowest (l) body weights] of Recessive White Rock (WRR) and Xinghua (XH) chickens to conduct integrative analyses of the miRNA-mRNA interactions involved in chicken skeletal muscle growth. A total of 162, 15, 173, and 27 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of the WRRh vs. XHh, WRRh vs. WRRl, WRRl vs. XHl, and XHh vs. XHl comparisons, respectively. Ingenuity Pathway Analysis revealed that gene networks identified for the WRRh vs. XHh contrast were associated with developmental disorders. Importantly, the WRRh vs. XHh contrast miRNA-mRNA network was enriched in IGF-1 signaling pathway genes, including FOXO3. A dual-luciferase reporter assay showed that FOXO3 was a target of miR-142-5p. Furthermore, miR-142-5p overexpression significantly decreased FOXO3 mRNA levels and promoted the expression of growth-related genes. These data demonstrated that miR-142-5p targets FOXO3 and promotes growth-related gene expression and regulates skeletal muscle growth in chicken. Comprehensive analysis facilitated the identification of miRNAs and target genes that might contribute to the regulation of skeletal muscle development. Our results provide new clues for understanding the molecular basis of chicken growth.

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