Cloning and characterization of WRKY gene homologs in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How) and their expression in response to fusaric acid treatment

文献类型： 外文期刊

第一作者： Mao, Yizhou

作者： Mao, Yizhou;Jiang, Biao;Peng, Qingwu;Liu, Wenrui;Lin, Yue;Xie, Dasen;He, Xiaoming;Mao, Yizhou;Jiang, Biao;Peng, Qingwu;Liu, Wenrui;Lin, Yue;Xie, Dasen;He, Xiaoming;Mao, Yizhou;Li, Shaoshan

作者机构：

关键词： Chieh-qua;Benincasa hispida;WRKY transcription factor;Fusarium resistance;Gene expression

期刊名称：3 BIOTECH （ 影响因子：2.406； 五年影响因子：3.203 ）

ISSN： 2190-572X

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： The WRKY transcription factors play an important role in plant resistance for biotic and abiotic stresses. In the present study, we cloned 10 WRKY gene homologs (CqWRKY) in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua) using the rapid-amplification of cDNA ends (RACE) or homology-based cloning methods. We characterized the structure of these CqWRKY genes. Phylogenetic analysis of these sequences with cucumber homologs suggested possible structural conservation of these genes among cucurbit crops. We examined the expression levels of these genes in response to fusaric acid (FA) treatment between resistant and susceptible Chieh-qua lines with quantitative real-time PCR. All genes could be upregulated upon FA treatment, but four CqWRKY genes exhibited differential expression between resistant and susceptible lines before and after FA application. CqWRKY31 seemed to be a positive regulator while CqWRKY1, CqWRKY23 and CqWRKY53 were negative regulators of fusaric resistance. This is the first report of characterization of WRKY family genes in Chieh-qua. The results may also be useful in breeding Chieh-qua for Fusarium wilt resistance.

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