MiR-34b-5p Suppresses Melanoma Differentiation-Associated Gene 5 (MDA5) Signaling Pathway to Promote Avian Leukosis Virus Subgroup J (ALV-J)-Infected Cells Proliferaction and ALV-J Replication

文献类型： 外文期刊

第一作者： Li, Zhenhui

作者： Li, Zhenhui;Luo, Qingbin;Xu, Haiping;Zheng, Ming;Abdalla, Bahareldin Ali;Feng, Min;Cai, Bolin;Zhang, Xiaocui;Nie, Qinghua;Zhang, Xiquan;Li, Zhenhui;Luo, Qingbin;Xu, Haiping;Zheng, Ming;Abdalla, Bahareldin Ali;Feng, Min;Cai, Bolin;Zhang, Xiaocui;Nie, Qinghua;Zhang, Xiquan;Li, Zhenhui;Luo, Qingbin;Xu, Haiping;Zheng, Ming;Abdalla, Bahareldin Ali;Feng, Min;Cai, Bolin;Zhang, Xiaocui;Nie, Qinghua;Zhang, Xiquan

作者机构：

关键词： MiR-34b-5p;melanoma differentiation-associated gene 5 (MDA5);Avian leukosis virus subgroup J (ALV-J);Cell proliferation;MDA5 signaling pathway

期刊名称：Frontiers in Cellular and Infection Microbiology （ 影响因子：5.293； 五年影响因子：5.882 ）

ISSN： 2235-2988

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Avian leukosis virus subgroup J (ALV-J) is an oncogenic retrovirus that has a similar replication cycle to multiple viruses and therefore can be used as a model system for viral entry into host cells. However, there are few reports on the genes or microRNAs (miRNAs) that are responsible for the replication of ALV-J. Our previous miRNA and RNA sequencing data showed that the expression of miR-34b-5p was significantly upregulated in ALV-J-infected chicken spleens compared to non-infected chicken spleens, but melanoma differentiation-associated gene 5 (MDA5) had the opposite expression pattern. In this study, a dual-luciferase reporter assay showed that MDA5 is a direct target of miR-34b-5p. In vitro, overexpression of miR-34b-5p accelerated the proliferation of ALV-J-infected cells by inducing the progression from G2 to S phase and it promoted cell migration. Ectopic expression of MDA5 inhibited ALV-J-infected cell proliferation, the cell cycle and cell migration, and knockdown of MDA5 promoted proliferation, the cell cycle and migration. In addition, during ALV-J infections, MDA5 can detect virus invasion and it triggers the MDA5 signaling pathway. MDA5 overexpression can activate the MDA5 signaling pathway, and thus it can inhibit the mRNA and protein expression of the ALV-J env gene and it can suppress virion secretion. In contrast, in response to the knockdown of MDA5 by small interfering RNA (siRNA) or an miR-34b-5p mimic, genes in the MDA5 signaling pathway were significantly downregulated (P < 0.05), but the mRNA and protein expression of ALV-J env and the sample-to-positive ratio of virion in the supernatants were increased. This indicates that miR-34b-5p is able to trigger the MDA5 signaling pathway and affect ALV-J infections. Together, these results suggest that miR-34b-5p targets MDA5 to accelerate the proliferation and migration of ALV-J-infected cells, and it promotes ALV-J replication, via the MDA5 signaling pathway.

分类号：

  • 相关文献

[1]Gp85 genetic diversity of avian leukosis virus subgroup J among different individual chickens from a native flock. Li, Yang,Fu, Jiayuan,Cui, Shuai,Meng, Fanfeng,Cui, Zhizhong,Chang, Shuang,Zhao, Peng,Fan, Jianhua.

[2]Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages. Xie, Xiaoli,Han, Meng,Zhang, Liang,Liu, Laixing,Gu, Zuye,Yang, Mei,Yang, Hongjun. 2016

[3]Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells. Gao, Chun-qi,Zhao, Yin-ling,Sui, Wei-guo,Yan, Hui-chao,Wang, Xiu-qi,Li, Hai-chang. 2015

[4]SMALL GRAIN 1, which encodes a mitogen-activated protein kinase kinase 4, influences grain size in rice. Duan, Penggen,Xu, Ran,Zhang, Baolan,Li, Yunhai,Duan, Penggen,Rao, Yuchun,Zeng, Dali,Yang, Yaolong,Dong, Guojun,Qian, Qian,Rao, Yuchun. 2014

[5]The multi-herbal formula Chong-Myung-Tang improves spatial memory and increases cell genesis in the dentate gyrus of aged mice. Liu, Lei,Zhang, Mingwei,Zhang, Ruifen,Lee, Mira,Hou, Jingang,Sung, Chang-Keun,Wang, Zhen. 2014

[6]Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens. Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan. 2018

[7]Proliferation and bone-related gene expression of osteoblasts grown on hydroxyapatite ceramics sintered at different temperature. Wang, CY,Duan, YR,Markovic, B,Barbara, J,Howlett, CR,Zhang, XD,Zreiqat, H. 2004

[8]Melatonin improves reprogramming efficiency and proliferation of bovine-induced pluripotent stem cells. Bai, Chunyu,Li, Xiangchen,Gao, Yuhua,Yuan, Ziao,Hu, Pengfei,Wang, Hui,Liu, Changqing,Guan, Weijun,Ma, Yuehui,Gao, Yuhua,Yuan, Ziao,Hu, Pengfei,Wang, Hui,Liu, Changqing. 2016

[9]Toxicarioside A, isolated from tropical Antiaris toxicaria, blocks endoglin/TGF-beta signaling in a bone marrow stromal cell line. Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Guo, Jun-Li,Tan, Guang-Hong. 2012

[10]Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99. Guo, HC,Liu, ZX,Sun, SQ,Bao, HF,Chen, YL,Liu, XT,Xie, QG.

[11]Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2. Huang, Y-C,Lee, C-H,Tsai, E-M,Yokoyama, K. K.,Pan, J.,Nakade, K.,Masuzaki, S.,Murata, T.,Yoshiki, A.,Obata, Y.,Yokoyama, K. K.,Pan, J.,Zhu, Z-W,Hasegawa, H.,Yamaguchi, N.,Lee, C-H,Yang, W-C,Yokoyama, K. K.. 2010

[12]Reticuloendotheliosis Virus Inhibits the Immune Response Acting on Lymphocytes from Peripheral Blood of Chicken. Bi, Yulin,Xu, Lu,Qiu, Lingling,Wang, Shasha,Zhang, Yani,Chen, Yang,Zhang, Yang,Xu, Qi,Chang, Guobin,Chen, Guohong,Liu, Xiangping. 2018

[13]The citrus flavonoid nobiletin inhibits proliferation and induces apoptosis in human pancreatic cancer cells in vitro. Zhang, Yu,Li, Hongjun,He, Zhifei,Zhang, Yu,Dou, Huating,Wu, Houjiu. 2014

[14]Chlorpyrifos is estrogenic and alters embryonic hatching, cell proliferation and apoptosis in zebrafish. Yu, Kaimin,Li, Guochao,Feng, Weimin,Liu, Lili,Zhang, Jiayu,Wu, Wei,Xu, Lei,Yan, Yanchun.

[15]Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina. Chen, Jiong-Ran,Yang, Zhi-Qiang,Yan, Zuo-Ting,Wang, Ling,Cui, Dong-An,Hu, Ting-Jun,Niu, Ting-Xian,Wang, Meng.

[16]Chicken gga-miR-130a targets HOXA3 and MDFIC and inhibits Marek's disease lymphoma cell proliferation and migration. Han, Bo,Lian, Ling,Li, Xin,Zhao, Chunfang,Qu, Lujiang,Yang, Ning,Liu, Changjun,Song, Jiuzhou.

[17]Anticancer effects of sweet potato protein on human colorectal cancer cells. Li, Peng-Gao,Mu, Tai-Hua,Deng, Le,Li, Peng-Gao,Li, Peng-Gao.

[18]08SG2/OsBAK1 regulates grain size and number, and functions differently in Indica and Japonica backgrounds in rice. Yuan, Hua,Fan, Shijun,Huang, Juan,Zhan, Shijie,Wang, Shifu,Gao, Peng,Chen, Weilan,Tu, Bin,Ma, Bingtian,Wang, Yuping,Qin, Peng,Li, Shigui,Yuan, Hua,Fan, Shijun,Zhan, Shijie,Wang, Shifu,Gao, Peng,Chen, Weilan,Tu, Bin,Ma, Bingtian,Wang, Yuping,Qin, Peng,Li, Shigui,Huang, Juan. 2017

[19]OsMAPK6, a mitogen-activated protein kinase, influences rice grain size and biomass production. Liu, Shuying,Hua, Lei,Dong, Sujun,Jiang, Jun'e,Zhang, Fang,Fang, Xiaohua,Chen, Fan,Chen, Hongqi,Zhu, Xudong,Li, Yunhai.

[20]Natural Variation in the Promoter of GSE5 Contributes to Grain Size Diversity in Rice. Duan, Penggen,Xu, Jinsong,Zhang, Baolan,Zhang, Guozheng,Huang, Ke,Huang, Luojiang,Xu, Ran,Li, Yunhai,Zeng, Dali,Qian, Qian,Geng, Mufan,Ge, Song,Zhang, Guozheng,Huang, Ke,Huang, Luojiang. 2017

作者其他论文 更多>>

微信小程序