The Long Intron 1 of Growth Hormone Gene from Reeves' Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines

文献类型： 外文期刊

第一作者： Liu, Wen-Sheng

作者： Liu, Wen-Sheng;Ma, Jing-E;Li, Wei-Xia;Zhang, Jin-Ge;Wang, Juan;Nie, Qing-Hua;Qiu, Feng-Fang;Fang, Mei-Xia;Zeng, Fang;Wang, Xing;Lin, Xi-Ran;Zhang, Li;Chen, Shao-Hao;Zhang, Xi-Quan;Liu, Wen-Sheng;Ma, Jing-E;Li, Wei-Xia;Zhang, Jin-Ge;Wang, Juan;Nie, Qing-Hua;Qiu, Feng-Fang;Fang, Mei-Xia;Zeng, Fang;Wang, Xing;Lin, Xi-Ran;Zhang, Li;Chen, Shao-Hao;Zhang, Xi-Quan;Liu, Wen-Sheng;Ma, Jing-E;Li, Wei-Xia;Zhang, Jin-Ge;Wang, Juan;Nie, Qing-Hua;Qiu, Feng-Fang;Fang, Mei-Xia;Zeng, Fang;Wang, Xing;Lin, Xi-Ran;Zhang, Li;Chen, Shao-Hao;Zhang, Xi-Quan;Liu, Wen-Sheng

作者机构：

关键词： turtle;pituitary gland;growth hormone gene;intron;expression

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2016 年 17 卷 4 期

页码：

收录情况： SCI

摘要： Turtles grow slowly and have a long lifespan. Ultrastructural studies of the pituitary gland in Reeves' turtle (Chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (GH) cells than other animal species in summer and winter. C. reevesii GH gene was cloned and species-specific similarities and differences were investigated. The full GH gene sequence in C. reevesii contains 8517 base pairs (bp), comprising five exons and four introns. Intron 1 was found to be much longer in C. reevesii than in other species. The coding sequence (CDS) of the turtle's GH gene, with and without the inclusion of intron 1, was transfected into four cell lines, including DF-1 chicken embryo fibroblasts, Chinese hamster ovary (CHO) cells, human embryonic kidney 293FT cells, and GH4C1 rat pituitary cells; the turtle growth hormone (tGH) gene mRNA and protein expression levels decreased significantly in the intron-containing CDS in these cell lines, compared with that of the corresponding intronless CDS. Thus, the long intron 1 of GH gene in Reeves' turtle might correlate with downregulated gene expression.

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